A systematic evaluation of miRNA:mRNA interactions involved in the migration and invasion of breast cancer cells

详细信息    查看全文

• 作者：Daya Luo (1) (2)
  James M Wilson (2)
  Nikki Harvel (2)
  Jimei Liu (2)
  Lirong Pei (2)
  Shuang Huang (2) (3)
  LesleyAnn Hawthorn (2) (4)
  Huidong Shi (2) (3)
  
• 关键词：Breast cancer ; MicroRNA target genes ; Migration ; Invasion
• 刊名：Journal of Translational Medicine
• 出版年：2013
• 出版时间：December 2013
• 年：2013
• 卷：11
• 期：1
• 全文大小：1312KB
• 参考文献：1. Bartel DP: MicroRNAs: target recognition and regulatory functions. / Cell 2009, 136:215鈥?33. CrossRef
  2. Ventura A, Jacks T: MicroRNAs and cancer: short RNAs go a long way. / Cell 2009, 136:586鈥?91. CrossRef
  3. Iorio MV, Ferracin M, Liu CG, Veronese A, Spizzo R, Sabbioni S, Magri E, Pedriali M, Fabbri M, Campiglio M: MicroRNA gene expression deregulation in human breast cancer. / Cancer Res 2005, 65:7065鈥?070. CrossRef
  4. Yu F, Yao H, Zhu P, Zhang X, Pan Q, Gong C, Huang Y, Hu X, Su F, Lieberman J, Song E: let-7 regulates self renewal and tumorigenicity of breast cancer cells. / Cell 2007, 131:1109鈥?123. CrossRef
  5. Valastyan S, Reinhardt F, Benaich N, Calogrias D, Szasz AM, Wang ZC, Brock JE, Richardson AL, Weinberg RA: A pleiotropically acting microRNA, miR-31, inhibits breast cancer metastasis. / Cell 2009, 137:1032鈥?046. CrossRef
  6. Ma L, Teruya-Feldstein J, Weinberg RA: Tumour invasion and metastasis initiated by microRNA-10b in breast cancer. / Nature 2007, 449:682鈥?88. CrossRef
  7. Ma L, Reinhardt F, Pan E, Soutschek J, Bhat B, Marcusson EG, Teruya-Feldstein J, Bell GW, Weinberg RA: Therapeutic silencing of miR-10b inhibits metastasis in a mouse mammary tumor model. / Nat Biotechnol 2010, 28:341鈥?47. CrossRef
  8. Kota J, Chivukula RR, O'Donnell KA, Wentzel EA, Montgomery CL, Hwang HW, Chang TC, Vivekanandan P, Torbenson M, Clark KR: Therapeutic microRNA delivery suppresses tumorigenesis in a murine liver cancer model. / Cell 2009, 137:1005鈥?017. CrossRef
  9. Steeg PS: Tumor metastasis: mechanistic insights and clinical challenges. / Nat Med 2006, 12:895鈥?04. CrossRef
  10. Valastyan S, Weinberg RA: Tumor Metastasis: Molecular Insights and Evolving Paradigms. / Cell 2011, 147:275鈥?92. CrossRef
  11. Sreekumar R, Sayan BS, Mirnezami AH, Sayan AE: MicroRNA control of invasion and metastasis pathways. / Frontiers Genet 2011, 2:58.
  12. Baranwal S, Alahari SK: miRNA control of tumor cell invasion and metastasis. / Int J Cancer 2010, 126:1283鈥?290.
  13. Su S, Li Y, Luo Y, Sheng Y, Su Y, Padia RN, Pan ZK, Dong Z, Huang S: Proteinase-activated receptor 2 expression in breast cancer and its role in breast cancer cell migration. / Oncogene 2009, 28:3047鈥?057. CrossRef
  14. McCarty KS Jr, Miller LS, Cox EB, Konrath J, McCarty KS Sr: Estrogen receptor analyses, Correlation of biochemical and immunohistochemical methods using monoclonal antireceptor antibodies. / Arch Pathol Lab Med 1985, 109:716鈥?21.
  15. Gregory PA, Bert AG, Paterson EL, Barry SC, Tsykin A, Farshid G, Vadas MA, Khew-Goodall Y, Goodall GJ: The miR-200 family and miR-205 regulate epithelial to mesenchymal transition by targeting ZEB1 and SIP1. / Nat Cell Biol 2008, 10:593鈥?01. CrossRef
  16. Wang F, Li Y, Zhou J, Xu J, Peng C, Ye F, Shen Y, Lu W, Wan X, Xie X: miR-375 Is Down-Regulated in Squamous Cervical Cancer and Inhibits Cell Migration and Invasion via Targeting Transcription Factor SP1. / Am J Pathol 2011, 179:2580鈥?588. CrossRef
  17. Neve RM, Chin K, Fridlyand J, Yeh J, Baehner FL, Fevr T, Clark L, Bayani N, Coppe J-P, Tong F: A collection of breast cancer cell lines for the study of functionally distinct cancer subtypes. / Cancer Cell 2006, 10:515鈥?27. CrossRef
  18. Hurst DR, Edmonds MD, Scott GK, Benz CC, Vaidya KS, Welch DR: Breast cancer metastasis suppressor 1 up-regulates miR-146, which suppresses breast cancer metastasis. / Cancer Res 2009, 69:1279鈥?283. CrossRef
  19. Enerly E, Steinfeld I, Kleivi K, Leivonen S-K, Aure MR, Russnes HG, Ronneberg JA, Johnsen H, Navon R, Rodland E: miRNA-mRNA Integrated Analysis Reveals Roles for miRNAs in Primary Breast Tumors. / PLoS One 2011, 6:e16915. CrossRef
  20. Genovesi LA, Carter KW, Gottardo NG, Giles KM, Dallas PB: Integrated Analysis of miRNA and mRNA Expression in Childhood Medulloblastoma Compared with Neural Stem Cells. / PLoS One 2011, 6:e23935. CrossRef
  21. Havelange V, Stauffer N, Heaphy CCE, Volinia S, Andreeff M, Marcucci G, Croce CM, Garzon R: Functional implications of microRNAs in acute myeloid leukemia by integrating microRNA and messenger RNA expression profiling. / Cancer 2011, 117:4696鈥?706. CrossRef
  22. Ma L, Huang Y, Zhu W, Zhou S, Zhou J, Zeng F, Liu X, Zhang Y, Yu J: An Integrated Analysis of miRNA and mRNA Expressions in Non-Small Cell Lung Cancers. / PLoS One 2011, 6:e26502. CrossRef
  23. Qin L-X: An Integrative Analysis of microRNA and mRNA Expression - A Case Study. / Cancer Informatics 2008, 6:0.
  24. Van der Auwera I, Limame R, van Dam P, Vermeulen PB, Dirix LY, Van Laere SJ: Integrated miRNA and mRNA expression profiling of the inflammatory breast cancer subtype. / Br J Cancer 2010, 103:532鈥?41. CrossRef
  25. Erler JT, Bennewith KL, Nicolau M, Dornhofer N, Kong C, Le QT, Chi JT, Jeffrey SS, Giaccia AJ: Lysyl oxidase is essential for hypoxia-induced metastasis. / Nature 2006, 440:1222鈥?226. CrossRef
  26. Chen LC, Tu SH, Huang CS, Chen CS, Ho CT, Lin HW, Lee CH, Chang HW, Chang CH, Wu CH: Human breast cancer cell metastasis is attenuated by lysyl oxidase inhibitors through down-regulation of focal adhesion kinase and the paxillin-signaling pathway. / Breast Cancer Res Treat 2012, 134:989鈥?004. CrossRef
  27. Turashvili G, Bouchal J, Baumforth K, Wei W, Dziechciarkova M, Ehrmann J, Klein J, Fridman E, Skarda J, Srovnal J: Novel markers for differentiation of lobular and ductal invasive breast carcinomas by laser microdissection and microarray analysis. / BMC Cancer 2007, 7:55. CrossRef
  28. Al-Alwan M, Olabi S, Ghebeh H, Barhoush E, Tulbah A, Al-Tweigeri T, Ajarim D, Adra C: Fascin Is a Key Regulator of Breast Cancer Invasion That Acts via the Modification of Metastasis-Associated Molecules. / PLoS One 2011, 6:e27339. CrossRef
  29. Li Y, Guo Z, Chen H, Dong Z, Pan ZK, Ding H, Su SB, Huang S: HOXC8-Dependent Cadherin 11 Expression Facilitates Breast Cancer Cell Migration through Trio and Rac. / Genes Cancer 2011, 2:880鈥?88. CrossRef
  30. Hurteau GJ, Spivack SD, Brock GJ: Potential mRNA Degradation Targets of hsa-miR-200c. / Cell Cycle 2006, 5:1951鈥?956. CrossRef
  31. Lonne G, Cornmark L, Zahirovic I, Landberg G, Jirstrom K, Larsson C: PKCalpha expression is a marker for breast cancer aggressiveness. / Mol Cancer 2010, 9:76. CrossRef
  32. Yue CH, Chiu YW, Tung JN, Tzang BS, Shiu JJ, Huang WH, Liu JY, Hwang JM: Expression of protein kinase C alpha and the MZF-1 and Elk-1 transcription factors in human breast cancer cells. / Chin J Physiol 2012, 55:31鈥?6.
  33. Chen RS, Song YM, Zhou ZY, Tong T, Li Y, Fu M, Guo XL, Dong LJ, He X, Qiao HX: Disruption of xCT inhibits cancer cell metastasis via the caveolin-1/[beta]-catenin pathway. / Oncogene 2008, 28:599鈥?09. CrossRef
  34. Gregory PA, Bracken CP, Smith E, Bert AG, Wright JA, Roslan S, Morris M, Wyatt L, Farshid G, Lim Y-Y: An autocrine TGF-beta/ZEB/miR-200 signaling network regulates establishment and maintenance of epithelial-mesenchymal transition. / Mol Biol Cell 2011, 22:1686鈥?698. CrossRef
  35. Korpal M, Ell BJ, Buffa FM, Ibrahim T, Blanco MA, Celia-Terrassa T, Mercatali L, Khan Z, Goodarzi H, Hua Y: Direct targeting of Sec23a by miR-200s influences cancer cell secretome and promotes metastatic colonization. / Nature medicine 2011, 17:1101鈥?108. CrossRef
  36. Bernstein BW, Bamburg JR: ADF/Cofilin: a functional node in cell biology. / Trends Cell Biol 2010, 20:187鈥?95. CrossRef
  
• 作者单位：Daya Luo (1) (2)
  James M Wilson (2)
  Nikki Harvel (2)
  Jimei Liu (2)
  Lirong Pei (2)
  Shuang Huang (2) (3)
  LesleyAnn Hawthorn (2) (4)
  Huidong Shi (2) (3)
  
  1. Medical College of Nanchang University, Nanchang, Jiangxi, PR China
  2. GHSU Cancer Center, Georgia Health Sciences University, Augusta, GA, USA
  3. Department of Biochemistry and Molecular Biology, Georgia Health Sciences University, Augusta, GA, USA
  4. Department of Pathology, Georgia Health Sciences University, Augusta, GA, USA
  

文摘

In this study we performed a systematic evaluation of functional miRNA-mRNA interactions associated with the invasiveness of breast cancer cells using a combination of integrated miRNA and mRNA expression profiling, bioinformatics prediction, and functional assays. Analysis of the miRNA expression identified 11 miRNAs that were differentially expressed, including 7 down-regulated (miR-200c, miR-205, miR-203, miR-141, miR-34a, miR-183, and miR-375) and 4 up-regulated miRNAs (miR-146a, miR-138, miR-125b1 and miR-100), in invasive cell lines when compared to normal and less invasive cell lines. Transfection of miR-200c, miR-205, and miR-375 mimics into MDA-MB-231 cells led to the inhibition of in vitro cell migration and invasion. The integrated analysis of miRNA and mRNA expression identified 35 known and novel target genes of miR-200c, miR-205, and mir-375, including CFL2, LAMC1, TIMP2, ZEB1, CDH11, PRKCA, PTPRJ, PTPRM, LDHB, and SEC23A. Surprisingly, the majority of these genes (27 genes) were target genes of miR-200c, suggesting that miR-200c plays a pivotal role in regulating the invasiveness of breast cancer cells. We characterized one of the target genes of miR-200c, CFL2, and demonstrated that CFL2 is overexpressed in aggressive breast cancer cell lines and can be significantly down-regulated by exogenous miR-200c. Tissue microarray analysis further revealed that CFL2 expression in primary breast cancer tissue correlated with tumor grade. The results obtained from this study may improve our understanding of the role of these candidate miRNAs and their target genes in relation to breast cancer invasiveness and ultimately lead to the identification of novel biomarkers associated with prognosis.