Genomics and relative expression analysis identifies key genes associated with high female to male flower ratio in Jatropha curcas L.
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- 作者：Manali Gangwar ; Hemant Sood ; Rajinder Singh Chauhan
- 关键词：cis ; regulatory elements ; Floral transition ; Female flowering ; Jatropha curcas ; Seed yield
- 刊名：Molecular Biology Reports
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：43
- 期：4
- 页码：305-322
- 全文大小：1,818 KB
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Hemant Sood (1)
Rajinder Singh Chauhan (1)
1. Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Waknaghat, 173234, Solan, Himachal Pradesh, India - 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Animal Anatomy, Morphology and Histology
Animal Biochemistry - 出版者：Springer Netherlands
- ISSN：1573-4978
文摘
Jatropha curcas, has been projected as a major source of biodiesel due to high seed oil content (42 %). A major roadblock for commercialization of Jatropha-based biodiesel is low seed yield per inflorescence, which is affected by low female to male flower ratio (1:25–30). Molecular dissection of female flower development by analyzing genes involved in phase transitions and floral organ development is, therefore, crucial for increasing seed yield. Expression analysis of 42 genes implicated in floral organ development and sex determination was done at six floral developmental stages of a J. curcas genotype (IC561235) with inherently higher female to male flower ratio (1:8–10). Relative expression analysis of these genes was done on low ratio genotype. Genes TFL1, SUP, AP1, CRY2, CUC2, CKX1, TAA1 and PIN1 were associated with reproductive phase transition. Further, genes CUC2, TAA1, CKX1 and PIN1 were associated with female flowering while SUP and CRY2 in female flower transition. Relative expression of these genes with respect to low female flower ratio genotype showed up to ~7 folds increase in transcript abundance of SUP, TAA1, CRY2 and CKX1 genes in intermediate buds but not a significant increase (~1.25 folds) in female flowers, thereby suggesting that these genes possibly play a significant role in increased transition towards female flowering by promoting abortion of male flower primordia. The outcome of study has implications in feedstock improvement of J. curcas through functional validation and eventual utilization of key genes associated with female flowering.