Expression of Toll-like receptor 2 is up-regulated in monocytes from patients with chronic obstructive pulmonary disease

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• 作者：Jaume Pons (1) (4)
  Jaume Sauleda (3)
  Verónica Regueiro (2)
  Carmen Santos (1)
  Meritxell López (3)
  Joana Ferrer (4)
  Alvar GN Agustí (2) (3)
  José A Bengoechea (1) (2)
  
• 刊名：Respiratory Research
• 出版年：2006
• 出版时间：December 2006
• 年：2006
• 卷：7
• 期：1
• 全文大小：968KB
• 参考文献：1. Celli BR, MacNee W: Standards for the diagnosis and treatment of patients with COPD: a summary of the ATS/ERS position paper. / Eur Respir J 2004, 23:932-46. CrossRef
  2. Gan WQ, Man SF, Senthilselvan A, Sin DD: Association between chronic obstructive pulmonary disease and systemic inflammation: a systematic review and a meta-analysis. / Thorax 2004, 59:574-80. CrossRef
  3. Sin DD, Man SF: Why are patients with chronic obstructive pulmonary disease at increased risk of cardiovascular diseases? The potential role of systemic inflammation in chronic obstructive pulmonary disease. / Circulation 2003, 107:1514-519. CrossRef
  4. Agusti AG, Sauleda J, Miralles C, Gomez C, Togores B, Sala E, Batle S, Busquets X: Skeletal muscle apoptosis and weight loss in chronic obstructive pulmonary disease. / Am J Respir Crit Care Med 2002, 166:485-89. CrossRef
  5. Agusti AG, Noguera A, Sauleda J, Sala E, Pons J, Busquets X: Systemic effects of chronic obstructive pulmonary disease. / Eur Respir J 2003, 21:347-60. CrossRef
  6. Sethi S, Evans N, Grant BJ, Murphy TF: New strains of bacteria and exacerbations of chronic obstructive pulmonary disease. / N Engl J Med 2002, 347:465-71. CrossRef
  7. White AJ, Gompertz S, Stockley RA: Chronic obstructive pulmonary disease . 6: The aetiology of exacerbations of chronic obstructive pulmonary disease. / Thorax 2003, 58:73-0. CrossRef
  8. Janeway CAJ, Medzhitov R: Innate immune recognition. / Annu Rev Immunol 2002, 20:197-16. CrossRef
  9. Akira S, Takeda K, Kaisho T: Toll-like receptors: critical proteins linking innate and acquired immunity. / Nat Immunol 2001, 2:675-80. CrossRef
  10. Van Amersfoort ES, Van Berkel TJ, Kuiper J: Receptors, mediators, and mechanisms involved in bacterial sepsis and septic shock. / Clin Microbiol Rev 2003, 16:379-14. CrossRef
  11. da Silva CJ, Soldau K, Christen U, Tobias PS, Ulevitch RJ: Lipopolysaccharide is in close proximity to each of the proteins in its membrane receptor complex. transfer from CD14 to TLR4 and MD-2. / J Biol Chem 2001, 276:21129-1135. CrossRef
  12. Monso E, Ruiz J, Rosell A, Manterola J, Fiz J, Morera J, Ausina V: Bacterial infection in chronic obstructive pulmonary disease. A study of stable and exacerbated outpatients using the protected specimen brush. / Am J Respir Crit Care Med 1995, 152:1316-320.
  13. Zalacain R, Sobradillo V, Amilibia J, Barron J, Achotegui V, Pijoan JI, Llorente JL: Predisposing factors to bacterial colonization in chronic obstructive pulmonary disease. / Eur Respir J 1999, 13:343-48. CrossRef
  14. Sethi S, Wrona C, Grant BJ, Murphy TF: Strain-specific immune response to Haemophilus influenzae in chronic obstructive pulmonary disease. / Am J Respir Crit Care Med 2004, 169:448-53. CrossRef
  15. Standardization of Spirometry, 1994 Update. American Thoracic Society / Am J Respir Crit Care Med 1995, 152:1107-136.
  16. Roca J, Sanchis J, Agusti-Vidal A, Segarra F, Navajas D, Rodriguez-Roisin R, Casan P, Sans S: Spirometric reference values from a Mediterranean population. / Bull Eur Physiopathol Respir 1986, 22:217-24.
  17. Hirschfeld M, Ma Y, Weis JH, Vogel SN, Weis JJ: Cutting edge: repurification of lipopolysaccharide eliminates signaling through both human and murine toll-like receptor 2. / J Immunol 2000, 165:618-22.
  18. Travassos LH, Girardin SE, Philpott DJ, Blanot D, Nahori MA, Werts C, Boneca IG: Toll-like receptor 2-dependent bacterial sensing does not occur via peptidoglycan recognition. / EMBO Rep 2004, 5:1000-006. CrossRef
  19. Deering RP, Orange JS: Development of a clinical assay to evaluate toll-like receptor function. / Clin Vaccine Immunol 2006, 13:68-6. CrossRef
  20. Cook DN, Pisetsky DS, Schwartz DA: Toll-like receptors in the pathogenesis of human disease. / Nat Immunol 2004, 5:975-79. CrossRef
  21. Sabroe I, Whyte MK, Wilson AG, Dower SK, Hubbard R, Hall I: Toll-like receptor (TLR) 4 polymorphisms and COPD. / Thorax 2004, 59:81.
  22. Bihl F, Salez L, Beaubier M, Torres D, Lariviere L, Laroche L, Benedetto A, Martel D, Lapointe JM, Ryffel B, Malo D: Overexpression of Toll-like receptor 4 amplifies the host response to lipopolysaccharide and provides a survival advantage in transgenic mice. / J Immunol 2003, 170:6141-150.
  23. Kalis C, Kanzler B, Lembo A, Poltorak A, Galanos C, Freudenberg MA: Toll-like receptor 4 expression levels determine the degree of LPS-susceptibility in mice. / Eur J Immunol 2003, 33:798-05. CrossRef
  24. Akira S: Mammalian Toll-like receptors. / Curr Opin Immunol 2003, 15:5-1. CrossRef
  25. Girardin SE, Travassos LH, Herve M, Blanot D, Boneca IG, Philpott DJ, Sansonetti PJ, Mengin-Lecreulx D: Peptidoglycan molecular requirements allowing detection by Nod1 and Nod2. / J Biol Chem 2003, 278:41702-1708. CrossRef
  26. Girardin SE, Boneca IG, Viala J, Chamaillard M, Labigne A, Thomas G, Philpott DJ, Sansonetti PJ: Nod2 is a general sensor of peptidoglycan through muramyl dipeptide (MDP) detection. / J Biol Chem 2003, 278:8869-872. CrossRef
  27. Riordan SM, Skinner N, Nagree A, McCallum H, McIver CJ, Kurtovic J, Hamilton JA, Bengmark S, Williams R, Visvanathan K: Peripheral blood mononuclear cell expression of toll-like receptors and relation to cytokine levels in cirrhosis. / Hepatology 2003, 37:1154-164. CrossRef
  28. Shuto T, Xu H, Wang B, Han J, Kai H, Gu XX, Murphy TF, Lim DJ, Li JD: Activation of NF-kappa B by nontypeable Hemophilus influenzae is mediated by toll-like receptor 2-TAK1-dependent NIK-IKK alpha /beta-I kappa B alpha and MKK3/6-p38 MAP kinase signaling pathways in epithelial cells. / Proc Natl Acad Sci U S A 2001, 98:8774-779. CrossRef
  29. Yoshimura A, Lien E, Ingalls RR, Tuomanen E, Dziarski R, Golenbock D: Cutting edge: recognition of Gram-positive bacterial cell wall components by the innate immune system occurs via Toll-like receptor 2. / J Immunol 1999, 163:1-.
  30. Li M, Carpio DF, Zheng Y, Bruzzo P, Singh V, Ouaaz F, Medzhitov RM, Beg AA: An essential role of the NF-kappa B/Toll-like receptor pathway in induction of inflammatory and tissue-repair gene expression by necrotic cells. / J Immunol 2001, 166:7128-135.
  31. Vabulas RM, Wagner H, Schild H: Heat shock proteins as ligands of toll-like receptors. / Curr Top Microbiol Immunol 2002, 270:169-84.
  32. Hogg JC, Chu F, Utokaparch S, Woods R, Elliott WM, Buzatu L, Cherniack RM, Rogers RM, Sciurba FC, Coxson HO, Pare PD: The nature of small-airway obstruction in chronic obstructive pulmonary disease. / N Engl J Med 2004, 350:2645-653. CrossRef
  33. Shuto T, Imasato A, Jono H, Sakai A, Xu H, Watanabe T, Rixter DD, Kai H, Andalibi A, Linthicum F, Guan YL, Han J, Cato AC, Lim DJ, Akira S, Li JD: Glucocorticoids synergistically enhance nontypeable Haemophilus influenzae-induced Toll-like receptor 2 expression via a negative cross-talk with p38 MAP kinase. / J Biol Chem 2002, 277:17263-7270. CrossRef
  34. Murphy TF, Brauer AL, Schiffmacher AT, Sethi S: Persistent colonization by Haemophilus influenzae in chronic obstructive pulmonary disease. / Am J Respir Crit Care Med 2004, 170:266-72. CrossRef
  35. Matsuguchi T, Musikacharoen T, Ogawa T, Yoshikai Y: Gene expressions of Toll-like receptor 2, but not Toll-like receptor 4, is induced by LPS and inflammatory cytokines in mouse macrophages. / J Immunol 2000, 165:5767-772.
  36. Sabroe I, Jones EC, Usher LR, Whyte MK, Dower SK: Toll-like receptor (TLR)2 and TLR4 in human peripheral blood granulocytes: a critical role for monocytes in leukocyte lipopolysaccharide responses. / J Immunol 2002, 168:4701-710.
  37. Noguera A, Busquets X, Sauleda J, Villaverde JM, MacNee W, Agusti AG: Expression of adhesion molecules and G proteins in circulating neutrophils in chronic obstructive pulmonary disease. / Am J Respir Crit Care Med 1998, 158:1664-668.
  38. Droemann D, Goldmann T, Tiedje T, Zabel P, Dalhoff K, Schaaf B: Toll-like receptor 2 expression is decreased on alveolar macrophages in cigarette smokers and COPD patients. / Respir Res 2005, 6:68. CrossRef
  39. Srivastava M, Jung S, Wilhelm J, Fink L, Buhling F, Welte T, Bohle RM, Seeger W, Lohmeyer J, Maus UA: The inflammatory versus constitutive trafficking of mononuclear phagocytes into the alveolar space of mice is associated with drastic changes in their gene expression profiles. / J Immunol 2005, 175:1884-893.
  40. Barnes PJ: New concepts in chronic obstructive pulmonary disease. / Annu Rev Med 2003, 54:113-29. CrossRef
  41. Barnes PJ, Ito K, Adcock IM: Corticosteroid resistance in chronic obstructive pulmonary disease: inactivation of histone deacetylase. / Lancet 2004, 363:731-33. CrossRef
  42. Agusti AG: Systemic effects of chronic obstructive pulmonary disease. / Novartis Found Symp 2001, 234:242-49. CrossRef
  43. Wick G, Knoflach M, Xu Q: Autoimmune and inflammatory mechanisms in atherosclerosis. / Annu Rev Immunol 2004, 22:361-03. CrossRef
  44. Heinrich PC, Behrmann I, Haan S, Hermanns HM, Muller-Newen G, Schaper F: Principles of interleukin (IL)-6-type cytokine signalling and its regulation. / Biochem J 2003, 374:1-0. CrossRef
  45. Wendling D, Racadot E, Wijdenes J: Treatment of severe rheumatoid arthritis by anti-interleukin 6 monoclonal antibody. / J Rheumatol 1993, 20:259-62.
  46. Shouda T, Yoshida T, Hanada T, Wakioka T, Oishi M, Miyoshi K, Komiya S, Kosai K, Hanakawa Y, Hashimoto K, Nagata K, Yoshimura A: Induction of the cytokine signal regulator SOCS3/CIS3 as a therapeutic strategy for treating inflammatory arthritis. / J Clin Invest 2001, 108:1781-788.
  
• 作者单位：Jaume Pons (1) (4)
  Jaume Sauleda (3)
  Verónica Regueiro (2)
  Carmen Santos (1)
  Meritxell López (3)
  Joana Ferrer (4)
  Alvar GN Agustí (2) (3)
  José A Bengoechea (1) (2)
  
  1. Unidad de Investigación, Hospital Son Dureta, Institut Universitari d'Investigacions en Ciències de la Salut (IUNICS), Palma Mallorca, Spain
  4. Servicio de Inmunología, Hospital Son Dureta, Palma Mallorca, Spain
  3. Servicio de Neumología, Hospital Son Dureta, Palma Mallorca, Spain
  2. Program Infection and Immunity, Fundació Caubet-CIMERA Illes Balears, Bunyola, Spain
  

文摘

Background Chronic obstructive pulmonary disease (COPD) is characterised by pulmonary and systemic inflammation which flare-up during episodes of acute exacerbation (AECOPD). Given the role of Toll-like receptors (TLRs) in the induction of inflammatory responses we investigated the involvement of TLRs in COPD pathogenesis. Methods The expression of TLR-2, TLR-4 and CD14 in monocytes was analyzed by flow cytometry. To study the functional responses of these receptors, monocytes were stimulated with peptidoglycan or lipopolysaccharide and the amounts of TNFα and IL-6 secreted were determined by ELISA. Results We found that the expression of TLR-2 was up-regulated in peripheral blood monocytes from COPD patients, either clinically stable or during AECOPD, as compared to never smokers or smokers with normal lung function. Upon stimulation with TLR-2 ligand monocytes from COPD patients secreted increased amounts of cytokines than similarly stimulated monocytes from never smokers and smokers. In contrast, the expressions of TLR-4 and CD14 were not significantly different between groups, and the response to lipopolysaccharide (a TLR-4 ligand) stimulation was not significantly different either. At discharge from hospital TLR-2 expression was down-regulated in peripheral blood monocytes from AECOPD patients. This could be due to the treatment with systemic steroids because, in vitro, steroids down-regulated TLR-2 expression in a dose-dependent manner. Finally, we demonstrated that IL-6, whose plasma levels are elevated in patients, up-regulated in vitro TLR-2 expression in monocytes from never smokers. Conclusion Our results reveal abnormalities in TLRs expression in COPD patients and highlight its potential relationship with systemic inflammation in these patients.