Effects of Septal Grafts on Acetylcholine Release from Rat Hippocampus After 192 IgG-Saporin Lesion

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• 作者：Michael Hilgert ; Joachim Hartmann ; Konrad L?ffelholz ; H¨¦l¨¨ne Jeltsch ; Jean-Christophe Cassel and Jochen Klein
• 关键词：192 IgG ; saporin ; acetylcholine ; hippocampus ; cholinergic dysfunction ; transplantation
• 刊名：Neurochemical Research
• 出版年：2003
• 出版时间：2003
• 年：2003
• 卷：28
• 期：3/4
• 页码：467-472
• 全文大小：1.41 MB

文摘

The cholinergic inputs to the rat hippocampus were lesioned by intraseptal injections of 192 IgG-saporin. After 15 days, fetal septal cells were grafted into the hippocampus. Thirteen months later, hippocampal acetylcholine (ACh) release was studied by microdialysis. Lesioning reduced basal ACh release (100 % ) to 20 % of normal, which was compensated for by the graft (71 % ). Infusion of the serotonin uptake inhibitor citalopram (100 M) enhanced ACh release to the same extent ( % of basal release) in all rat groups. Systemic injection of 8-OH-DPAT (0.5 mg/kg, SC), an agonist of 5-HT_1A receptors, caused a smaller ACh release than citalopram. Acetylcholinesterase (AChE) staining and densitometric quantification revealed that the lesion-induced reduction of the AChE-staining density was compensated for by septal grafting. In conclusion, both histochemical and biochemical methods showed that cholinergic hippocampal parameters were drastically impaired by 192 IgG-saporin lesions, but were almost completely restored by septal grafting. The graft responded to intrinsic serotonergic regulation.