Harnessing anthocyanin-rich fruit: a visible reporter for tracing virus-induced gene silencing in pepper fruit

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• 作者：Jihyun Kim ; Minkyu Park ; Eun Soo Jeong ; Je Min Lee ; Doil Choi
• 关键词：VIGS ; Pepper ; TRV ; Reporter system ; An2 ; Anthocyanin ; Capsaicinoid
• 刊名：Plant Methods
• 出版年：2017
• 出版时间：December 2017
• 年：2017
• 卷：13
• 期：1
• 全文大小：1742KB
• 刊物主题：Plant Sciences; Biological Techniques;
• 出版者：BioMed Central
• ISSN：1746-4811
• 卷排序：13

文摘

BackgroundVirus-induced gene silencing (VIGS) has become a powerful tool for post-genomic technology in plant species. This is important, especially in select plants, such as the pepper plant, that are recalcitrant to Agrobacterium-mediated transformation. Although VIGS in plants has been widely employed as a powerful tool for functional genomics, scattering phenotypic effects by uneven gene silencing has been implemented in order to overcome challenges in experiments with fruit tissues.