Towards an integrated device that utilizes adherent cells in a micro-free-flow electrophoresis chip to achieve separation and biosensing

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• 作者：Stefan Jezierski (1)
  Anke S. Klein (2)
  Christian Benz (1)
  Michael Schaefer (2)
  Stefan Nagl (1)
  Detlev Belder (1)
  
• 关键词：Free ; flow electrophoresis ; Adenosine triphosphate ; Microfluidic ; Cell response
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2013
• 出版时间：June 2013
• 年：2013
• 卷：405
• 期：16
• 页码：5381-5386
• 全文大小：313KB
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• 作者单位：Stefan Jezierski (1)
  Anke S. Klein (2)
  Christian Benz (1)
  Michael Schaefer (2)
  Stefan Nagl (1)
  Detlev Belder (1)
  
  1. Institut für Analytische Chemie, Universit?t Leipzig, Johannisallee 29, 04103, Leipzig, Germany
  2. Rudolf-Boehm-Institut für Pharmakologie und Toxikologie, Universit?t Leipzig, H?rtelstr. 16-18, 04107, Leipzig, Germany
  
• ISSN：1618-2650

文摘

We immobilized adherent human embryonic kidney (HEK) cells—which are able to trace adenosine triphosphate (ATP) —inside a microfluidic free-flow electrophoresis (μFFE) chip in order to develop an integrated device combining separation and biosensing capabilities. HEK 293 cells loaded with fluorescent calcium indicators were used as a model system to enable the spatially and temporally resolved detection of ATP. The local position of a 20?μM ATP stream was successfully visualized by these cells during free-flow electrophoresis, demonstrating the on-line detection capability of this technique towards native, unlabeled compounds.