The RNA-binding properties and domain of Rice stripe virus nucleocapsid protein

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• 作者：Shuling Zhao ; Yanan Xue ; Jiahui Hao ; Changyong Liang
• 关键词：Rice stripe virus ; Nucleocapsid protein ; EMSA ; Binding domain
• 刊名：Virus Genes
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：51
• 期：2
• 页码：276-282
• 全文大小：1,086 KB
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• 作者单位：Shuling Zhao (1)
  Yanan Xue (1)
  Jiahui Hao (1)
  Changyong Liang (1)
  
  1. College of Bioscience and Biotechnology, Yangzhou University, No. 12 Wenhui Road East, Yangzhou, 225009, People’s Republic of China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Medical Microbiology
  Virology
  Plant Sciences
  
• 出版者：Springer Netherlands
• ISSN：1572-994X

文摘

The nucleocapsid protein (NP) of rice stripe virus (RSV) encapsidates viral genomic RNAs to form virion. The binding of NP with RNA is essential for the formation of virus particle. In this study, the binding specificity of RSV NP to RNA and the domains within the NP that mediate this interaction were investigated by gel electrophoretic mobility shift assays and Northwestern blot analysis. The results demonstrated that RSV NP was able to bind to all synthetic RNAs and DNAs without sequence specificity. Using a series of truncated NPs expressed in E. coli and synthetic peptides, we mapped the RNA-binding domain of NP to the central region from amino acid residues 201-32. Further alanine substitution analysis revealed that Lys206, Lys207, Lys220, and Tyr221 in the RNA-binding domain were essential for NP to bind with RNA. Keywords Rice stripe virus Nucleocapsid protein EMSA Binding domain