Archaeal DNA polymerases in biotechnology

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• 作者：Likui Zhang ; Manyu Kang ; Jiajun Xu ; Yanchao Huang
• 关键词：Archaeal DNA pols ; PCR ; DNA sequencing ; Site ; directed mutagenesis ; Fidelity ; Processivity
• 刊名：Applied Microbiology and Biotechnology
• 出版年：2015
• 出版时间：August 2015
• 年：2015
• 卷：99
• 期：16
• 页码：6585-6597
• 全文大小：1,564 KB
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• 作者单位：Likui Zhang (1)
  Manyu Kang (1)
  Jiajun Xu (1)
  Yanchao Huang (1)
  
  1. Marine Science & Technology Institute, Department of Environmental Science and Engineering, Yangzhou University, No. 196 Huayang West Road, Hanjiang District, Yangzhou City, 225127, Jiangsu Province, China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Microbiology
  Microbial Genetics and Genomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-0614

文摘

DNA polymerase (pol) is a ubiquitous enzyme that synthesizes DNA strands in all living cells. In vitro, DNA pol is used for DNA manipulation, including cloning, PCR, site-directed mutagenesis, sequencing, and several other applications. Family B archaeal DNA pols have been widely used for molecular biological methods. Biochemical and structural studies reveal that each archaeal DNA pol has different characteristics with respect to fidelity, processivity and thermostability. Due to their high fidelity and strong thermostability, family B archaeal DNA pols have the extensive application on high-fidelity PCR, DNA sequencing, and site-directed mutagenesis while family Y archaeal DNA pols have the potential for error-prone PCR and random mutagenesis because of their low fidelity and strong thermostability. This information combined with mutational analysis has been used to construct novel DNA pols with altered properties that enhance their use as biotechnological reagents. In this review, we focus on the development and use of family B archaeal DNA pols.