Magnetic nanoparticles are highly toxic to chloroquine-resistant Plasmodium falciparum, dengue virus (DEN-2), and their mosquito vectors

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• 作者：Kadarkarai Murugan ; Jiang Wei ; Mohamad Saleh Alsalhi…
• 关键词：Antiviral activity ; Malaria ; Magnetospirillum gryphiswaldense ; Magnetotactic bacteria ; Yellow fever ; Zika virus
• 刊名：Parasitology Research
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：116
• 期：2
• 页码：495-502
• 全文大小：726KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Medical Microbiology; Microbiology; Immunology;
• 出版者：Springer Berlin Heidelberg
• ISSN：1432-1955
• 卷排序：116

文摘

A main challenge in parasitology is the development of reliable tools to prevent or treat mosquito-borne diseases. We investigated the toxicity of magnetic nanoparticles (MNP) produced by Magnetospirillum gryphiswaldense (strain MSR-1) on chloroquine-resistant (CQ-r) and sensitive (CQ-s) Plasmodium falciparum, dengue virus (DEN-2), and two of their main vectors, Anopheles stephensi and Aedes aegypti, respectively. MNP were studied by Fourier-transform infrared spectroscopy and transmission electron microscopy. They were toxic to larvae and pupae of An. stephensi, LC₅₀ ranged from 2.563 ppm (1st instar larva) to 6.430 ppm (pupa), and Ae. aegypti, LC₅₀ ranged from 3.231 ppm (1st instar larva) to 7.545 ppm (pupa). MNP IC₅₀ on P. falciparum were 83.32 μg ml−1 (CQ-s) and 87.47 μg ml−1 (CQ-r). However, the in vivo efficacy of MNP on Plasmodium berghei was low if compared to CQ-based treatments. Moderate cytotoxicity was detected on Vero cells post-treatment with MNP doses lower than 4 μg ml−1. MNP evaluated at 2–8 μg ml−1 inhibited DEN-2 replication inhibiting the expression of the envelope (E) protein. In conclusion, our findings represent the first report about the use of MNP in medical and veterinary entomology, proposing them as suitable materials to develop reliable tools to combat mosquito-borne diseases.