Functional TRPV and TRPM channels in human preadipocytes
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- 作者：Hui Che (1)
Jianbo Yue (2)
Hung-Fat Tse (1)
Gui-Rong Li (1) (2) - 关键词：Human preadipocytes ; Transient receptor potential channels ; Proliferation ; Adipogenesis ; shRNA
- 刊名：Pfl篓鹿gers Archiv - European Journal of Physiology
- 出版年：2014
- 出版时间：May 2014
- 年：2014
- 卷：466
- 期：5
- 页码：947-959
- 全文大小：
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Jianbo Yue (2)
Hung-Fat Tse (1)
Gui-Rong Li (1) (2)
1. Department of Medicine, Li-Ka Shing Faculty of Medicine, University of Hong Kong, Laboratory Block, FMB, 21 Sassoon Road, Pokfulam, Hong Kong, China
2. Department of Physiology, Li-Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong, China - ISSN：1432-2013
文摘
Preadipocytes are widely used as an in vitro model to investigate proliferation, adipogenic differentiation, and lipodystrophy; however, cellular physiology and biology are not fully understood in human preadipocytes. The present study was to investigate the expression of transient receptor potential (TRP) channels in human preadipocytes and their potential roles in regulating proliferation and adipogenic differentiation using approaches of confocal microscopy, whole-cell patch voltage-clamp, reverse transcription polymerase chain reaction, Western blot, etc. We found that TRPV2, TRPV4, and TRPM7 channels were abundantly expressed in human preadipocytes. The intracellular Ca2+ transient activated by the TRPV2 activator probenecid was reversed or prevented by ruthenium red, a TRPV2 blocker. The TRPV4 channel activator, 4α-phorbol 12-13-dicaprinate, enhanced intracellular Ca2+ oscillations, and the effect was inhibited by the TRPV4 blocker RN-1734. TRPM7 current was recorded with dialysis of Mg2+-free pipette solution, which was inhibited by the TRP channel blocker 2-aminoethoxydiphenyl borate and enhanced by acidic extracellular pH. Silencing TRPV2 or TRPM7, but not TRPV4, significantly reduced cell proliferation via inhibiting cyclin D1, cyclin E, and p-ERK1/2. Interestingly, individually silencing these three channels decreased adipogenic differentiation of human preadipocytes by reducing p-Akt kinase. Our results demonstrate for the first time that functional TRPV2, TRPV4, and TRPM7 channels are abundantly expressed in human preadipocytes. TRPV2 and TRPM7, but not TRPV4, regulate cell proliferation via activating cyclin D1, cyclin E, and p-ERK1/2, while they are all involved in adipogenesis in human preadipocytes via phosphorylating Akt kinase.