Development of twelve nuclear microsatellites in the endemic herb Rehmannia chingii using next generation sequencing

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• 作者：Yuan Ma ; Chunyan Liu ; Zhong-Hu Li ; Jianfang Li…
• 关键词：Rehmannia chingii ; Microsatellites ; Illumina ; Genetic diversity
• 刊名：Conservation Genetics Resources
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：7
• 期：1
• 页码：223-224
• 全文大小：149 KB
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• 刊物主题：Conservation Biology/Ecology; Plant Sciences; Animal Anatomy / Morphology / Histology; Evolutionary Biology;
• 出版者：Springer Netherlands
• ISSN：1877-7260

文摘

Rehmannia chingii, an endemic and rare medicine herb, has been seriously threatened by human activities. Here, we developed twelve microsatellites by using next generation sequencing method to evaluate its genetic diversity. The number of alleles per locus varied from two to four. The expected (H E ) and observed heterozygosity (H O ) and fixation indices per locus ranged from 0.122 to 0.418, from 0.133 to 0.600 and from ?.091 to ?.442, with an average of 0.275, 0.353 and ?.238, respectively. No significant linkage disequilibrium and deviation from Hardy–Weinberg equilibrium were detected. These polymorphic microsatellite markers will be helpful for the conservation and restoration of this endangered plant.