Genetic fine mapping and candidate gene analysis of the Gossypium hirsutum Ligon lintless-1 (Li1) mutant on chromosome 22(D)

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• 作者：Yurong Jiang ; Mingquan Ding ; Yuefen Cao ; Fen Yang…
• 关键词：Cotton ; Fiber ; Ligon lintless ; 1 ; Genetic mapping ; Single ; strand conformation polymorphism (SSCP) marker
• 刊名：Molecular Genetics and Genomics
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：290
• 期：6
• 页码：2199-2211
• 全文大小：1,236 KB
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• 作者单位：Yurong Jiang (1) (2)
  Mingquan Ding (1)
  Yuefen Cao (1)
  Fen Yang (1)
  Hua Zhang (1)
  Shae He (1)
  Huaqin Dai (1)
  Huanfeng Hao (1)
  Junkang Rong (1) (2)
  
  1. The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, School of Agriculture and Food Science, Zhejiang A&F University, Linan, Hangzhou, 311300, Zhejiang, China
  2. State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, 455000, Henan, China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Cell Biology
  Biochemistry
  Microbial Genetics and Genomics
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1617-4623

文摘

Ligon lintless-1 (Li1) is a Gossypium hirsutum mutant that is controlled by a dominant gene that arrests the development of cotton fiber after anthesis. Two F₂ mapping populations were developed from mutant (Li1 × H7124) F₁ plants in 2012 and 2013; each was composed of 142 and 1024 plants, respectively. Using these populations, Li1 was mapped to a 0.3-cM region in which nine single-strand conformation polymorphism markers co-segregated with the Li1 locus. In the published G. raimondii genome, these markers were mapped to a region of about 1.2 Mb (the Li1 region) and were separated by markers that flanked the Li1 locus in the genetic map, dividing the Li1 region into three segments. Thirty-six genes were annotated by the gene prediction software FGENESH (Softberry) in the Li1 region. Twelve genes were candidates of Li1, while the remaining 24 genes were identified as transposable elements, DNA/RNA polymerase superfamily or unknown function genes. Among the 12 candidate genes, those encoding ribosomal protein s10, actin protein, ATP synthase, and beta-tubulin 5 were the most-promising candidates of the Li1 mutant because the function of these genes is closely related to fiber development. High-throughput RNA sequencing and quantitative PCR revealed that these candidate genes had obvious differential gene expression between mutant and wild-type plants at the fiber elongation stage, strengthening the inference that they could be the most likely candidate gene of the Li1 mutant phenotype. Keywords Cotton Fiber Ligon lintless-1 Genetic mapping Single-strand conformation polymorphism (SSCP) marker