Activating transcription factor 5 regulates lipid metabolism in adipocytes
详细信息 查看全文
- 作者：Jing-Hui Jiang ; Yue Zhao ; Liu-Ling Xiao ; Cui-Song Zhu ; Shu-Fen Li…
- 关键词：Activating transcription factor 5 ; Adipocytes ; Lipid metabolism ; Fatty acid synthetase ; Stearyl coenzyme A desaturation enzyme 1 ; Carnitine palmitoyl transferase 1
- 刊名：Science Bulletin
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：61
- 期：23
- 页码：1802-1809
- 全文大小：2297KB
- 刊物主题：Science, Humanities and Social Sciences, multidisciplinary; Life Sciences, general; Physics, general; Chemistry/Food Science, general; Earth Sciences, general; Engineering, general;
- 出版者：Science China Press
- ISSN：2095-9281
- 卷排序：61
文摘
Activating transcription factor 5 (ATF5) is a member of the activating transcription factor/cAMP response element binding protein (ATF/CREB) family, and is highly expressed in liver and adipose tissue. Previous reports have shown that ATF5 promoted 3T3-L1 preadipocytes differentiation. In this study, we found that ATF5 was highly expressed in mature adipocytes, suggesting a potential role of ATF5 in mature adipocytes, which has not been reported previously. To understand the function of ATF5 in mature adipocytes, we knocked down the expression of ATF5 in 3T3-L1 mature adipocytes and observed decreased lipid droplets. Consistent with the in vitro experiment, the knockdown of ATF5 in white adipose tissue led to less adipose tissue and smaller adipocytes size. Further research revealed that the inhibition of ATF5 diminished the adipocytes size via the inhibition of fatty acid synthetase, stearyl coenzyme A desaturation enzyme 1, and the induction of carnitine palmitoyl transferase 1, one key enzyme of lipid metabolism. In addition, ATF5 knockdown in inguinal white adipose tissue improved whole body insulin sensitivity. Our work provides a new understanding of ATF5 function in mature adipocytes and a potential therapeutic target of diabetes.