Rat liver sinusoidal surface N-linked glycoproteomic analysis by affinity enrichment and mass spectrometric identification

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• 作者：Jianglin Li (1)
  Jun Gao (2)
  Miao Jiang (1)
  Jia Chen (1)
  Zhonghua Liu (1)
  Ping Chen (1)
  Songping Liang (1)
  
  1. Key Laboratory of Protein Chemistry and Developmental Biology of the Ministry of Education ; College of Life Sciences ; Hunan Normal University ; Changsha ; 410081 ; P. R. China
  2. Qingyuan City People鈥檚 Hospital of Jinan University ; Qingyuan ; Guangdong ; 511518 ; P. R. China
  
• 关键词：liver sinusoidal endothelial surface ; N ; glycoproteomic ; N ; glyco ; FASP ; mass spectrometry
• 刊名：Biochemistry (Moscow)
• 出版年：2015
• 出版时间：March 2015
• 年：2015
• 卷：80
• 期：3
• 页码：260-275
• 全文大小：1,084 KB
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• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Biochemistry
  Bioorganic Chemistry
  Microbiology
  Biomedicine
  Russian Library of Science
  
• 出版者：MAIK Nauka/Interperiodica distributed exclusively by Springer Science+Business Media LLC.
• ISSN：1608-3040

文摘

Glycosylation in liver is one of the most biologically important protein modifications. It plays critical roles in many physiological and pathological processes by virtue of its unique location at the blood-tissue interface, including angiogenesis, liver cancer, cirrhosis, and fibrosis. To analyze glycosylation of plasma membrane proteins in liver sinusoidal endothelial cells (LSEC), N-glycopeptides of the LSEC surface were enriched using a filter-assisted sample preparation-based lectin affinity capture method and subsequently identified with mass spectrometry. In total, 225 unique N-glycosylation sites on 152 glycoproteins were identified, of which 119 (53%) sites had not previously been determined experimentally. Among the glycoproteins, 53% were classified as plasma membrane proteins and 47 (31%) as signaling proteins and receptors. Moreover, 23 cluster of differentiation antigens with 49 glycopeptides were detected within the membrane glycoproteins of the liver sinusoidal surface. Furthermore, bioinformatics analysis revealed that the majority of identified glycoproteins have an impact on processes of LSEC. Therefore, N-glycoproteomic analysis of the liver sinusoidal surface may provide useful information on liver regeneration and facilitate liver disease diagnosis.