Proteomics analysis of plasma membrane from liver sinusoidal endothelial cells after partial hepatectomy by an improved two-dimensional electrophoresis
详细信息 查看全文
- 作者：Xuanwen Li ; Li Xiong ; Chunliang Xie ; Jia Cao ; Huobao Deng ; Yong Lin ; Rui Cao ; Jianglin Li ; Ping Chen and Songping Liang
- 关键词：16 ; BAC ; PAGE ; Angiogenesis ; Partial hepatectomy ; Plasma membrane ; Proteomics
- 刊名：Molecular and Cellular Biochemistry
- 出版年：2010
- 出版时间：November 2010
- 年：2010
- 卷：344
- 期：1-2
- 页码：137-150
- 全文大小：1.3 MB
文摘
Liver regeneration is an angiogenesis-associated phenomenon. To identify key plasma membrane (PM) proteins of endothelial cells involved in the initiation of angiogenesis during liver regeneration, the PM of liver sinusoidal endothelial cells (LSEC) at 72 h after partial hepatectomy was enriched by an established in vivo membrane density perturbation method. The differentially expressed membrane proteins compared to those from sham operation were quantified using an improved two-dimensional 16-BAC/SDS-PAGE and identified by LC-MS/MS. Several proteins were further confirmed by cICAT labeling quantitative strategy. A total of 47 proteins were identified including known and novel proteins involved in angiogenesis or liver regeneration, such as inducible nitric oxide synthase, type IV collagen, and integrin beta3. Our results indicated that the combination of the membrane density perturbation strategy and the improved two-dimensional electrophoresis (2-DE) method are useful for investigating the endothelial dysfunctions in vivo.