Development of real-time and nested RT-PCR to detect astrovirus and one-year survey of astrovirus in Jiangmen City, China

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• 作者：Ying-chun Dai ; Qiao-hua Xu ; Xian-bo Wu ; Gui-fang Hu ; Ya-li Tang ; Jian-dong Li ; Qing Chen and Jun Nie
• 关键词：Human astrovirus ; Real ; time RT ; PCR ; Nested RT ; PCR ; Genetic analysis ; Acute gastroenteritis ; Children ; Adults
• 刊名：Archives of Virology
• 出版年：2010
• 出版时间：June, 2010
• 年：2010
• 卷：155
• 期：6
• 页码：977-982
• 全文大小：231.4 KB

文摘

Human astroviruses (HAstVs) are one of the major viral agents of acute gastroenteritis (AGE) in all age groups, especially in young children. In this study, new one-step real-time RT-PCR and nested RT-PCR assays were developed to detect HAstVs. HAstVs were identified in 46 (8.75 % ) of 526 stool samples in Jiangmen City over 1 year, including 43 (9.15 % ) of 470 children and 3 (5.4 % ) of 56 adults, and HAstV-1 was the most predominant strain. This finding suggests that HAstVs infections are common in Jiangmen City, China. Further detailed molecular epidemiological studies are required for understanding the prevalence of HAstVs infection and gaining knowledge about the circulating genotypes.