Generating Hypoimmunogenic Human Embryonic Stem Cells by the Disruption of Beta 2-Microglobulin

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• 作者：Pengfei Lu (1) (2)
  Jijun Chen (1) (2)
  Lixiazi He (1) (2)
  Jiangtao Ren (1)
  Haide Chen (2) (3)
  Lingjun Rao (1)
  Qinggang Zhuang (1)
  Hui Li (4)
  Lei Li (1)
  Lei Bao (1) (2)
  Ji He (5)
  Wei Zhang (5)
  Faming Zhu (5)
  Chun Cui (2) (3)
  Lei Xiao (2) (3)
  
• 关键词：Beta 2 ; microglobulin ; Human leukocyte antigens ; Hypoimmunogenicity ; Human embryonic stem cells ; Immune rejection ; iPS cells
• 刊名：Stem Cell Reviews and Reports
• 出版年：2013
• 出版时间：December 2013
• 年：2013
• 卷：9
• 期：6
• 页码：806-813
• 全文大小：
• 作者单位：Pengfei Lu (1) (2)
  Jijun Chen (1) (2)
  Lixiazi He (1) (2)
  Jiangtao Ren (1)
  Haide Chen (2) (3)
  Lingjun Rao (1)
  Qinggang Zhuang (1)
  Hui Li (4)
  Lei Li (1)
  Lei Bao (1) (2)
  Ji He (5)
  Wei Zhang (5)
  Faming Zhu (5)
  Chun Cui (2) (3)
  Lei Xiao (2) (3)
  
  1. Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, People’s Republic of China
  2. Zhejiang University School of Medicine, 866 Yuhangtang Road, Nongshenghuan Building E#, Room 301, Hangzhou, 310058, People’s Republic of China
  3. Stem Cell and Developmental Biology Research Center, College of Animal Science, Zhejiang University, Hangzhou, 310058, People’s Republic of China
  4. Xiangtan Center Hospital, Hunan, 411100, People’s Republic of China
  5. Blood Centre of Zhejiang Province, Hangzhou, 310058, People’s Republic of China
  
• ISSN：1558-6804

文摘

Immune rejection hinders the application of human embryonic stem cells (hESCs) in transplantation therapy. Human leukocyte antigens (HLAs) on the cell surface are the major cause of graft rejection. In this study, we generated HLA class I-deficient hESCs via disruption of beta 2-microglobulin (β2m), the light chain of HLA Class I. We found that HLA class I proteins were not present on the cell surface of β2m-null hESCs. These cells showed the same pluripotency as wildtype hESCs and demonstrated hypoimmunogenicity. Thus, HLA class I-deficient hESCs might serve as an unlimited cell source for the generation of universally compatible “off-the-shelf” cell grafts, tissues or organs in the future.