Highly Efficient Derivation of Skeletal Myotubes from Human Embryonic Stem Cells

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• 作者：Lingjun Rao (1) (3)
  Wenjie Tang (2)
  Youzhen Wei (2)
  Lei Bao (1) (3)
  Jijun Chen (1) (3)
  Haide Chen (3)
  Lixiazi He (1) (3)
  Pengfei Lu (1) (3)
  Jiangtao Ren (1) (3)
  Lu Wu (1) (3)
  Zhidong Luan (4)
  Chun Cui (3)
  Lei Xiao (3)
  
• 关键词：Human embryonic stem cells ; Skeletal myotube ; Directed differentiation ; Muscle development ; Tet ; on ; MyoD
• 刊名：Stem Cell Reviews and Reports
• 出版年：2012
• 出版时间：December 2012
• 年：2012
• 卷：8
• 期：4
• 页码：1109-1119
• 全文大小：889KB
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• 作者单位：Lingjun Rao (1) (3)
  Wenjie Tang (2)
  Youzhen Wei (2)
  Lei Bao (1) (3)
  Jijun Chen (1) (3)
  Haide Chen (3)
  Lixiazi He (1) (3)
  Pengfei Lu (1) (3)
  Jiangtao Ren (1) (3)
  Lu Wu (1) (3)
  Zhidong Luan (4)
  Chun Cui (3)
  Lei Xiao (3)
  
  1. Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, People’s Republic of China
  3. Stem Cell and Developmental Biology Research Center, College of Animal Science, Zhejiang University, 866 Yuhangtang Road, Nongshenghuan Building E#, Room 301, Hangzhou, 310058, People’s Republic of China
  2. Shanghai East Hospital, Tongji University, Shanghai, 200120, People’s Republic of China
  4. Department of Developmental Biology, Liaoning Medical University, Jinzhou, 121001, People’s Republic of China
  

文摘

Human embryonic stem cells (hESCs) are a promising model for the research of embryonic development and regenerative medicine. Since the first hESC line was established, many researchers have shown that pluripotent hESCs can be directed into many types of functional adult cells in culture. However, most of the reported methods have induced differentiation through the alteration of growth factors in the culture medium. These methods are time consuming; moreover, it is difficult to obtain a pure population of the desired cells because of the low efficiency of induction. In this study, we used a lentiviral-based inducible gene-expression system in hESCs to control the ectopic expression of MyoD, which is an essential transcription factor in skeletal muscle development. The induction of MyoD can efficiently direct the pluripotent hESCs into mesoderm in 24?h. The cells then become proliferated myoblasts and finally form multinucleated myotubes in vitro. The whole procedure took about 10?days, with an induction efficiency of over 90?%. To our knowledge, this is the first time that hESCs have been induced into terminally differentiated cells with only one factor. In the future, these results could be a potential resource for cell therapy for diseases of muscle dysfunction.