Mitochondrial FOXO3a is involved in amyloid β peptide-induced mitochondrial dysfunction

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• 作者：Chun Shi ; Jianhua Zhu ; Shuilong Leng…
• 刊名：Journal of Bioenergetics and Biomembranes
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：48
• 期：3
• 页码：189-196
• 全文大小：1,467 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Bioorganic Chemistry
  Biochemistry
  Animal Anatomy, Morphology and Histology
  Animal Biochemistry
  Organic Chemistry
  
• 出版者：Springer New York
• ISSN：1573-6881
• 卷排序：48

文摘

Mitochondrial dysfunction is a hallmark of amyloid β peptide (Aβ)-induced neuronal toxicity in Alzheimer’s disease (AD). However, the precise mechanism(s) of Aβ-induced mitochondrial dysfunction has not been fully understood. There is evidence that Forkhead box O3a (FOXO3a) is normally present in neuronal mitochondria. Using HT22 murine hippocampal neuronal cells and primary hippocampal neurons, the present study investigated whether mitochondrial FOXO3a was involved in mitochondrial dysfunction induced by Aβ. It was found that Aβ induced dephosphorylation and mitochondrial translocation of FOXO3a. In addition, Aβ enhanced association of FOXO3a with mitochondrial DNA (mtDNA), causing a decrease in the expression of cytochrome c oxidase subunit 1 (COX1) and the activity of COX. In addition, Aβ-induced mitochondrial dysfunction, indicated by the decrease in 3- (4,5-cimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) conversion, mitochondrial adenosine triphosphate (ATP) production and COX activity, could be suppressed by knockdown of FOXO3a (FOXO3a-KD). These results provide new insights into the mechanism underlying Aβ-induced neurotoxicity and open up new therapeutic perspectives for AD.KeywordsFOXO3aAβMitochondriaCOX1