Naringin Protects Against Cartilage Destruction in Osteoarthritis Through Repression of NF-κB Signaling Pathway

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• 作者：Yunpeng Zhao ; Zhong Li ; Wenhan Wang ; Hui Zhang ; Jianying Chen ; Peng Su…
• 关键词：TNF ; α ; osteoarthritis ; chondrocyte ; naringin ; NF ; κB signaling
• 刊名：Inflammation
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：39
• 期：1
• 页码：385-392
• 全文大小：4,225 KB
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• 作者单位：Yunpeng Zhao (1)
  Zhong Li (2)
  Wenhan Wang (3)
  Hui Zhang (3)
  Jianying Chen (4)
  Peng Su (3)
  Long Liu (3)
  Weiwei Li (3)
  
  1. Department of Orthopaedics, Qilu Hospital, Shandong University, 107 Wenhuaxi Road, Jinan, Shandong, 250012, People’s Republic of China
  2. Department of Orthopaedics, Liaocheng Clinical Academy, Taishan Medical University, Liaocheng, Shandong, 252000, People’s Republic of China
  3. Department of Pathology, Qilu Hospital, Shandong University, 107 Wenhuaxi Road, Jinan, Shandong, 250012, People’s Republic of China
  4. Institute of Biopharmaceuticals of Shandong Province, Jinan, Shandong, 250101, People’s Republic of China
  
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Rheumatology
  Internal Medicine
  Pharmacology and Toxicology
  Pathology
  
• 出版者：Springer Netherlands
• ISSN：1573-2576

文摘

Naringin was previously reported as a multifunctional agent. Recently, naringin was found to play a protective role in various inflammatory conditions. However, the role of naringin in cartilage degeneration and osteoarthritis (OA) progression is still unknown. TNF-α is reported to play a detrimental role in OA. Herein, primary murine chondrocytes were isolated and cultured with stimulation of TNF-α, in the presence or absence of naringin treatment. As a result, naringin attenuated TNF-α-mediated inflammation and catabolism in chondrocyte. Besides, surgically induced OA mice models were established. Cartilage degradation and OA severity were evaluated using Safranin-O staining, immunohistochemistry, and ELISA. Moreover, levels of inflammatory cytokines and catabolic markers in OA were analyzed. Oral administration of naringin alleviated degradation of cartilage matrix and protected against OA development in the surgically induced OA models. Furthermore, the protective function of naringin in cartilage and chondrocyte was possibly due to suppression of NF-κB signaling pathway. Collectively, this study presents naringin as a potential target for the treatment of joint degenerative diseases, including OA. KEY WORDS TNF-α osteoarthritis chondrocyte naringin NF-κB signaling