Improvement of FK506 production by selection of 4-Aminobutyric acid-tolerant mutant and optimization of its fermentation using response surface methodology

详细信息    查看全文

• 作者：Tianyi Yang (1)
  Jiaojiao Li (1)
  Liangzhi Li (1) (2)
  Haiyang Zhang (1)
  Jing Ma (1)
  Zhi Chen (1)
  Cuiying Hu (1)
  Xin Ju (1)
  Jiaolong Fu (1)
  
• 关键词：4 ; Aminobutyric acid ; mutant ; response surface methodology ; Streptomyces tsukubaensis ; tacrolimus
• 刊名：Journal of the Korean Society for Applied Biological Chemistry
• 出版年：2014
• 出版时间：December 2014
• 年：2014
• 卷：57
• 期：6
• 页码：715-722
• 全文大小：293 KB
• 参考文献：1. Chen D, Zhang Q, Zhang Q, Cen P, Xu Z, and Liu W (2012) Improvement of FK506 production in Streptomyces tsukubaensis by genetic enhancement of the supply of unusual polyketide extender units via utilization of two distinct site-specific recombination systems. / Appl Environ Microbiol 78, 5093鈥?03. CrossRef
  2. Choudhari S and Singhal R (2008) Media optimization for the production of beta-carotene by Blakeslea trispora: a statistical approach. / Bioresour Technol 99, 722鈥?0. CrossRef
  3. Goranovic D, Bla啪ic M, Magdevska V, Horvat J, Ku拧cer E, Polak T, Santos-Aberturas J, Mart铆nez-Castro M, Barreiro C, Mrak P, Kopitar G, Kosec G, Fujs S, Mart铆n JF, and Petkovi忙 H (2012) FK506 biosynthesis is regulated by two positive regulatory elements in Streptomyces tsukubaensis. / BMC Microbiol 12, 238. CrossRef
  4. Goranovic D, Kosec G, Mrak P, Fujs S, Horvat J, Kuscer E, Kopitar G, and Petkovic H (2010) Origin of the allyl group in FK506 biosynthesis. / J Biol Chem 285, 14292鈥?00. CrossRef
  5. Huang D, Li S, Xia M, Wen J, and Jia X (2013) Genome-scale metabolic network guided engineering of Streptomyces tsukubaensis for FK506 production improvement. / Microb Cell Fact 12, 52. CrossRef
  6. Kim HS and Park YI (2007) Lipase activity and tacrolimus production in Streptomyces clavuligerus CKD 1119 mutant strains. / J Microbiol Biotechnol 17, 1638鈥?4.
  7. Kim SY, Lee KH, Kim JH, and Oh DK (1997) Erythritol production by controlling osmotic pressure in Trigonopsis variabilis. / Biotechnol Lett 19, 727鈥?. CrossRef
  8. Kino T, Hatanaka H, Hashimoto M, Nishiyama M, Goto T, Okuhara M, Kohsaka M, and Aoki H (1987) Imanaka H. FK-506, a novel immunosuppressant isolated from a Streptomyces. I. Fermentation, isolation, and physico-chemical and biological characteristics. / J Antibiot 40, 1249鈥?5. CrossRef
  9. Kumar P, Malviya HK, Maurya RK, and Shukla A (2007) Fermentation processes for the preparation of FK506. International publication patent no. WO 2007/039816 A2, USA.
  10. Kumar P, Sharma S, Shukla A, Kumar S, Maurya RK, Katial V, Mitra A, and Gigras P (2005) Production of tacrolimus (FK-506) using new Streptomyces species. WO 2005/038009 A2, USA.
  11. Li S, Xu H, Yu JG, Wang YY, Feng XH, and Ouyang PK (2013) Enhancing isomaltulose production by recombinant / Escherichia coli producing sucrose isomerase: culture medium optimization containing agricultural wastes and cell immobilization. / Bioprocess Biosyst Eng 36, 1395鈥?05. CrossRef
  12. Manivasagan P, Sivasankar P, Venkatesan J, Sivakumar K, and Kim SK (2014) Optimization, production and characterization of glycolipid biosurfactant from the marine actinobacterium, Streptomyces sp. MAB36. / Bioprocess Biosyst Eng 37, 783鈥?7. CrossRef
  13. Mart铆nez-Castro M, Salehi-Najafabadi Z, Romero F, P茅rez-Sanchiz R, Fern谩ndez-Chimeno RI, Mart铆n JF, and Barreiro C (2013) Taxonomy and chemically semi-defined media for the analysis of the tacrolimus producer 鈥楽treptomyces tsukubaensis鈥? / Appl Microbiol Biotechnol 97, 2139鈥?2. CrossRef
  14. Mo S, Ban YH, Park JW, Yoo YJ, and Yoon YJ (2009) Enhanced FK506 production in Streptomyces clavuligerus CKD1119 by engineering the supply of methylmalonyl-CoA precursor. / J Ind Microbiol Biotechnol 36, 1473鈥?2. CrossRef
  15. Mo S, Kim DH, Lee JH, Park JW, Basnet DB, Ban YH, Yoo YJ, Chen SW, Park SR, Choi EA, Kim E, Jin YY, Lee SK, Park JY, Liu Y, Lee MO, Lee KS, Kim SJ, Kim D, Park BC, Lee SG, Kwon HJ, Suh JW, Moore BS, Lim SK, and Yoon YJ (2011) Biosynthesis of the allylmalonyl-CoA extender unit for the FK506 polyketide synthase proceeds through a dedicated polyketide synthase and facilitates the mutasynthesis of analogues. / J Am Chem Soc 133, 976鈥?5. CrossRef
  16. Mo S, Lee SK, Jin YY, Oh CH, and Suh JW (2013) Application of a combined approach involving classical random mutagenesis and metabolic engineering to enhance FK506 production in Streptomyces sp. RM7011. / Appl Microbiol Biotechnol 97, 3053鈥?2. CrossRef
  17. Mo S, Yoo YJ, Ban YH, Lee SK, Kim E, Suh JW, and Yoon YJ (2012) Roles of fkbN in positive regulation and tcs7 in negative regulation of FK506 biosynthesis in Streptomyces sp. strain KCTC 11604BP. / Appl Environ Microbiol 78, 2249鈥?5. CrossRef
  18. Motamedi H, Cai SJ, Shafiee A, and Elliston KO (1997) Structural organization of a multifunctional polyketide synthase involved in the biosynthesis of the macrolide immunosuppressant FK506. / Eur J Biochem 244, 74鈥?0. CrossRef
  19. Oh DK, Cho CH, Lee JK, and Kim SY (2001) Increased erythritol production in fed-batch cultures of Torula sp. by controlling glucose concentration. / J Ind Microbiol Biotechnol 26, 248鈥?2. CrossRef
  20. Parsons WH, Sigal NH, and Wyvratt MJ (1993) FK-506 a novel immunosuppressant. / Ann N Y Acad Sci 685, 22鈥?6. CrossRef
  21. Singh BP and Behera BK (2009) Regulation of tacrolimus production by altering primary source of carbons and amino acids. / Lett Appl Microbiol 49, 254鈥?. CrossRef
  22. Streeter JG (1972) In Vivo and In Vitro Studies on gamma-Aminobutyric Acid Metabolism with the Radish Plant (Raphanus sativus, L.). / Plant Physiol 49, 579鈥?4. CrossRef
  23. Thomson AW (1990) FK-506 enters the clinic. / Immunol Today 11, 35鈥?. CrossRef
  24. Turlo J, Gajzlerska W, Klimaszewska M, Kr贸l M, Dawidowski M, and Gutkowska B (2012) Enhancement of tacrolimus productivity in Streptomyces tsukubaensis by the use of novel precursors for biosynthesis. / Enzyme Microb Technol 51, 388鈥?5. CrossRef
  25. Vaid S and Narula P (2006) Novel process for producing FK506 (FK-506) using vegetable oil as sole source of carbon. International publication patent no. WO 2006/011156 A2, USA.
  
• 作者单位：Tianyi Yang (1)
  Jiaojiao Li (1)
  Liangzhi Li (1) (2)
  Haiyang Zhang (1)
  Jing Ma (1)
  Zhi Chen (1)
  Cuiying Hu (1)
  Xin Ju (1)
  Jiaolong Fu (1)
  
  1. School of Chemistry and Bioengineering, Suzhou University of Science and Technology, Suzhou, 215009, P. R. China
  2. Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin, 300072, P. R. China
  
• ISSN：2234-344X

文摘

Tacrolimus (FK506), a 23-membered polyketide macrolide with immunosuppressant activity, can be produced by Streptomyces tsukubaensis. We studied a variety of mutant strains of S. tsukubaensis for the microbial production of FK506. The best strain (CZ-19) was obtained from the parent strain LLZ-1 by nitrosoguanidine mutation and 4-Aminobutyric acid (FK506 precursor structure analogs) adaption. In the shake-flask experiments, titer of FK506 by CZ-19 was 532.44 mg/L, increased by 65.13% compared to that of the parent strain. Through single factor experiments and response surface methodology, we further optimized the medium for improved FK506 production by CZ-19 in shake flask culture. The optimal medium for enhanced FK506 production was as follows: 17.19 g/L corn starch, 21.78 g/L glucose, 8.06 g/L peptone, and 18.98 mg/L 4-aminobutyric acid. The predicted FK506 titer was 906.49 mg/L, and the experimental data confirmed the validity of the model. The present study demonstrates that S. tsukubaensis CZ-19 is a promising strain for industrial production of FK506.