A functional peptidoglycan hydrolase characterized from T4SS in 89K pathogenicity island of epidemic Streptococcus suis serotype 2
详细信息 查看全文
- 作者：Qiu Zhong (17) (18)
Yan Zhao (17)
Tian Chen (17)
Supeng Yin (17)
Xinyue Yao (17)
Jing Wang (17)
Shuguang Lu (17)
Yinling Tan (17)
Jiaqi Tang (19)
Beiwen Zheng (20)
Fuquan Hu (17)
Ming Li (17) - 关键词：Streptococcus suis serotype 2 ; Streptococcal toxic shock syndrome ; Type IV secretion system ; Pathogenicity island ; Assembly ; Peptidoglycan hydrolase
- 刊名：BMC Microbiology
- 出版年：2014
- 出版时间：December 2014
- 年：2014
- 卷：14
- 期：1
- 全文大小：555 KB
- 参考文献：1. Gottschalk M, Xu J, Calzas C, Segura M: Streptococcus suis : a new emerging or an old neglected zoonotic pathogen? / Future Microbiol 2010,5(3):371鈥?91. CrossRef
2. Segura M: Streptococcus suis : an emerging human threat. / J Infect Dis 2009,199(1):4鈥?. CrossRef
3. Feng Y, Zhang H, Ma Y, Gao GF: Uncovering newly emerging variants of Streptococcus suis , an important zoonotic agent. / Trends Microbiol 2010,18(3):124鈥?31. CrossRef
4. Huang YT, Teng LJ, Ho SW, Hsueh PR: Streptococcus suis infection. / J Microbiol Immunol Infect 2005,38(5):306鈥?13.
5. Sriskandan S, Slater JD: Invasive disease and toxic shock due to zoonotic Streptococcus suis : an emerging infection in the East? / PLoS Med 2006,3(5):e187. CrossRef
6. Gottschalk M, Segura M, Xu J: Streptococcus suis infections in humans: the Chinese experience and the situation in North America. / Anim Health Res Rev 2007,8(1):29鈥?5. CrossRef
7. Tang J, Wang C, Feng Y, Yang W, Song H, Chen Z, Yu H, Pan X, Zhou X, Wang H, Wu B, Wang H, Zhao H, Lin Y, Yue J, Wu Z, He X, Gao F, Khan AH, Wang J, Zhao G, Wang Y, Wang X, Chen Z, Gao GF: Streptococcal toxic shock syndrome caused by Streptococcus suis serotype 2. / PLoS Med 2006,3(5):e151. CrossRef
8. Yu H, Jing H, Chen Z, Zheng H, Zhu X, Wang H, Wang S, Liu L, Zu R, Luo L, Xiang N, Liu H, Liu X, Shu Y, Lee SS, Chuang SK, Wang Y, Xu J, Yang W, Streptococcus suis study groups: Human Streptococcus suis outbreak, Sichuan, China. / Emerg Infect Dis 2006,12(6):914鈥?20. CrossRef
9. Breiman RFDJ, Facklam RR, Gray BM, Hoge CW, Kaplan EL, Mortimer EA, Schlievert PM, Schwartz B, Stevens DL, Todd JK: Defining the group A streptococcal toxic shock syndrome: rationale and consensus definition: the working group on severe streptococcal infections. / Jama 1993,269(3):390鈥?91. CrossRef
10. Chen C, Tang J, Dong W, Wang C, Feng Y, Wang J, Zheng F, Pan X, Liu D, Li M, Song Y, Zhu X, Sun H, Feng T, Guo Z, Ju A, Ge J, Dong Y, Sun W, Jiang Y, Wang J, Yan J, Yang H, Wang X, Gao GF, Yang R, Wang J, Yu J: A glimpse of streptococcal toxic shock syndrome from comparative genomics of S. suis 2 Chinese isolates. / PLoS ONE 2007, 2:e315. CrossRef
11. Li M, Wang C, Feng Y, Pan X, Cheng G, Wang J, Ge J, Zheng F, Cao M, Dong Y, Liu D, Wang J, Lin Y, Du H, Gao GF, Wang X, Hu F, Tang J: SalK/SalR, a two-component signal transduction system, is essential for full virulence of highly invasive Streptococcus suis serotype 2. / PLoS One 2008,3(5):e2080. CrossRef
12. Li M, Shen X, Yan J, Han H, Zheng B, Liu D, Cheng H, Zhao Y, Rao X, Wang C, Tang J, Hu F, Gao GF: GI-type T4SS-mediated horizontal transfer of the 89K pathogenicity island in epidemic Streptococcus suis serotype 2. / Mol Microbiol 2011,79(6):1670鈥?683. CrossRef
13. Zhao Y, Liu G, Li S, Wang M, Song J, Wang J, Tang J, Li M, Hu F: Role of a type IV-like secretion system of Streptococcus suis 2 in the development of streptococcal toxic shock syndrome. / J Infect Dis 2011,204(2):274鈥?81. CrossRef
14. Alvarez-Martinez CE, Christie PJ: Biological diversity of prokaryotic type IV secretion systems. / Microbiol Mol Biol Rev 2009,73(4):775鈥?08. CrossRef
15. Abajy MY, Kopec J, Schiwon K, Burzynski M, Doring M, Bohn C, Grohmann E: A type IV-secretion-like system is required for conjugative DNA transport of broad-host-range plasmid pIP501 in gram-positive bacteria. / J Bacteriol 2007,189(6):2487鈥?496. CrossRef
16. Grohmann E, Muth G, Espinosa M: Conjugative plasmid transfer in gram-positive bacteria. / Microbiol Mol Biol Rev 2003,67(2):277鈥?01. table of contents CrossRef
17. Zahrl D, Wagner M, Bischof K, Bayer M, Zavecz B, Beranek A, Ruckenstuhl C, Zarfel GE, Koraimann G: Peptidoglycan degradation by specialized lytic transglycosylases associated with type III and type IV secretion systems. / Microbiology 2005,151(Pt 11):3455鈥?467. CrossRef
18. Fronzes R, Christie PJ, Waksman G: The structural biology of type IV secretion systems. / Nat Rev Microbiol 2009,7(10):703鈥?14. CrossRef
19. Bateman A, Rawlings ND: The CHAP domain: a large family of amidases including GSP amidase and peptidoglycan hydrolases. / Trends Biochem Sci 2003,28(5):234鈥?37. CrossRef
20. Rigden DJ, Jedrzejas MJ, Galperin MY: Amidase domains from bacterial and phage autolysins define a family of gamma-D, L-glutamate-specific amidohydrolases. / Trends Biochem Sci 2003,28(5):230鈥?34. CrossRef
21. Donovan DM, Foster-Frey J, Dong S, Rousseau GM, Moineau S, Pritchard DG: The cell lysis activity of the Streptococcus agalactiae bacteriophage B30 endolysin relies on the cysteine, histidine-dependent amidohydrolase/peptidase domain. / Appl Environ Microbiol 2006,72(7):5108鈥?112. CrossRef
22. Frankel MB, Hendrickx AP, Missiakas DM, Schneewind O: LytN, a murein hydrolase in the cross-wall compartment of Staphylococcus aureus , is involved in proper bacterial growth and envelope assembly. / J Biol Chem 2011,286(37):32593鈥?2605. CrossRef
23. Baron C, Llosa M, Zhou S, Zambryski PC: VirB1, a component of the T-complex transfer machinery of Agrobacterium tumefaciens , is processed to a C-terminal secreted product, VirB1. / J Bacteriol 1997,179(4):1203鈥?210.
24. Blackburn NT, Clarke AJ: Assay for lytic transglycosylases: a family of peptidoglycan lyases. / Anal Biochem 2000,284(2):388鈥?93. CrossRef
25. Mushegian AR, Fullner KJ, Koonin EV, Nester EW: A family of lysozyme-like virulence factors in bacterial pathogens of plants and animals. / Proc Natl Acad Sci U S A 1996,93(14):7321鈥?326. CrossRef
26. Holtje JV, Mirelman D, Sharon N, Schwarz U: Novel type of murein transglycosylase in Escherichia coli . / J Bacteriol 1975,124(3):1067鈥?076.
27. Koraimann G: Lytic transglycosylases in macromolecular transport systems of Gram-negative bacteria. / Cell Mol Life Sci 2003,60(11):2371鈥?388. CrossRef
28. Arends K, Celik EK, Probst I, Goessweiner-Mohr N, Fercher C, Grumet L, Soellue C, Abajy MY, Sakinc T, Broszat M, Schiwon K, Koraimann G, Keller W, Grohmann E: TraG encoded by the pIP501 type IV secretion system is a two-domain peptidoglycan-degrading enzyme essential for conjugative transfer. / J Bacteriol 2013,195(19):4436鈥?444. CrossRef
29. Mao J, Schmelcher M, Harty WJ, Foster-Frey J, Donovan DM: Chimeric Ply187 endolysin kills Staphylococcus aureus more effectively than the parental enzyme. / FEMS Microbiol Lett 2013,342(1):30鈥?6. CrossRef
30. Berger BR, Christie PJ: Genetic complementation analysis of the Agrobacterium tumefaciens virB operon: virB2 through virB11 are essential virulence genes. / J Bacteriol 1994,176(12):3646鈥?660.
31. Zhong Q, Shao S, Mu R, Wang H, Huang S, Han J, Huang H, Tian S: Characterization of peptidoglycan hydrolase in Cag pathogenicity island of Helicobacter pylori . / Mol Biol Rep 2011,38(1):503鈥?09. CrossRef
32. Leber TM, Balkwill FR: Zymography: a single-step staining method for quantitation of proteolytic activity on substrate gels. / Anal Biochem 1997,249(1):24鈥?8. CrossRef
33. Strating H, Clarke AJ: Differentiation of bacterial autolysins by zymogram analysis. / Anal Biochem 2001,291(1):149鈥?54. CrossRef
34. Clarke AJ: Extent of peptidoglycan O acetylation in the tribe Proteeae . / J Bacteriol 1993,175(14):4550鈥?553.
35. Yuan Y, Peng Q, Gao M: Characteristics of a broad lytic spectrum endolysin from phage BtCS33 of Bacillus thuringiensis . / BMC Microbiol 2012, 12:297. CrossRef
36. Wang H, Shen X, Zhao Y, Wang M, Zhong Q, Chen T, Hu F, Li M: Identification and proteome analysis of the two-component VirR/VirS system in epidemic Streptococcus suis serotype 2. / FEMS Microbiol Lett 2012,333(2):160鈥?68. CrossRef - 作者单位：Qiu Zhong (17) (18)
Yan Zhao (17)
Tian Chen (17)
Supeng Yin (17)
Xinyue Yao (17)
Jing Wang (17)
Shuguang Lu (17)
Yinling Tan (17)
Jiaqi Tang (19)
Beiwen Zheng (20)
Fuquan Hu (17)
Ming Li (17)
17. Department of Microbiology, Third Military Medical University, Chongqing, 400038, China
18. Department of Scientific Research, Daping Hospital, Research Institute of Surgery, Third Military Medical University, Chongqing, 400042, China
19. PLA Research Institute of Clinical Laboratory Medicine, Nanjing general hospital of Nanjing Military command, Nanjing, 210002, China
20. State Key Laboratory for Diagnosis and Treatment of Infectious Disease, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, 310003, China - ISSN：1471-2180
文摘
Background Streptococcus suis serotype 2 (S. suis 2) has evolved efficient mechanisms to cause streptococcal toxic shock syndrome (STSS), which is a new emerging infectious disease linked to S. suis. We have previously reported that a type IV secretion system (T4SS) harbored by the specific 89K pathogenicity island (PAI) of S. suis 2 contributes to the development of STSS and mediates horizontal transfer of 89K. However, the 89K T4SS machinery assembly in vivo and in vitro is poorly understood, and the component acting directly to digest the bacterial cell wall needs to be identified. Results The virB1-89K gene product encoded in the 89K PAI is the only one that shows similarity to the Agrobacterium VirB1 component and contains a conserved CHAP domain that may function in peptidoglycan hydrolysis, which makes it a plausible candidate acting as a hydrolase against the peptidoglycan cell wall to allow the assembly of the T4SS apparatus. In the current study, the CHAP domain of VirB1-89K from S. suis 89K PAI was cloned and over-expressed in Escherichia coli, and its peptidoglycan-degrading activity in vitro was determined. The results indicated that the VirB1-89K CHAP domain can degrade the peptidoglycan layer of bacteria. Deletion of virB1-89K reduces significantly, but does not abolish, the virulence of S. suis in a mouse model. Conclusions The experimental results presented here suggested that VirB1-89K facilitates the assembly of 89K T4SS apparatus by catalyzing the degradation of the peptidoglycan cell wall, thus contributing to the pathogenesis of S. suis 2 infection.