Evaluation of a DNA vaccine candidate co-expressing GP3 and GP5 of porcine reproductive and respiratory syndrome virus (PRRSV) with interferon α/γ in immediate and long-lasting protection against HP-PRRSV challenge

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• 作者：Yijun Du (1)
  Jing Qi (1)
  Yu Lu (2)
  Jiaqiang Wu (1)
  Dongwan Yoo (3)
  Xing Liu (1)
  Xiumei Zhang (1)
  Jun Li (1)
  Wenbo Sun (1)
  Xiaoyan Cong (1)
  Jianli Shi (1)
  Jinbao Wang (1)
  
• 关键词：PRRSV ; GP3 ; GP5 ; Interferon α/γ ; Immune responses
• 刊名：Virus Genes
• 出版年：2012
• 出版时间：December 2012
• 年：2012
• 卷：45
• 期：3
• 页码：474-487
• 全文大小：686KB
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• 作者单位：Yijun Du (1)
  Jing Qi (1)
  Yu Lu (2)
  Jiaqiang Wu (1)
  Dongwan Yoo (3)
  Xing Liu (1)
  Xiumei Zhang (1)
  Jun Li (1)
  Wenbo Sun (1)
  Xiaoyan Cong (1)
  Jianli Shi (1)
  Jinbao Wang (1)
  
  1. Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Sangyuan Road No.10, Jinan, 250100, China
  2. National Research Center of Veterinary Biologicals Engineering and Technology, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China
  3. Department of Pathobiology, University of Illinois at Urbana-Champaign, 2001 South Lincoln Ave, Urbana, IL, 61802, USA
  
• ISSN：1572-994X

文摘

Porcine reproductive and respiratory syndrome virus (PRRSV) has become one of the most economically important diseases to the global pork industry. Current vaccination strategies only provide a limited protective efficacy. In this study, a DNA vaccine, pVAX1?-α-γ-GP35, co-expressing GP3 and GP5 of PRRSV with interferon α/γ was constructed, and its immediate and long-lasting protection against highly pathogenic PRRSV (HP-PRRSV) challenge were examined in pigs. For immediate protection, the results showed that pVAX1?-α-γ-GP35 could provide partially protective efficacy, which was similar to the pVAX1?-α-γ (expressing interferon α/γ). For long-lasting protection, pigs inoculated with pVAX1?-α-γ-GP35 developed significantly higher PRRSV-specific antibody response, T cell proliferation, IFN-γ, and IL-4, than those vaccinated with pVAX1?-GP35 (expressing GP3 and GP5 of PRRSV). Following homologous challenge with HP-PRRSV strain SD-JN, pigs inoculated with pVAX1?-α-γ-GP35 showed almost no clinical signs, no lung lesions, and significantly lower viremia, as compared to those in pVAX1?-GP35 group. It indicated that pVAX1?-α-γ-GP35 could induce enhanced immune responses and provide both immediate and long-lasting protection against HP-PRRSV challenge in pigs. The DNA vaccine pVAX1?-α-γ-GP35 might be an attractive candidate vaccine for the prevention and control of HP-PRRSV infections.