Expression of cytochrome P450 CYP81A6 in rice: tissue specificity, protein subcellular localization, and response to herbicide application

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• 作者：Hai-ping Lu (1)
  Martin Edwards (2)
  Qi-zhao Wang (1)
  Hai-jun Zhao (1)
  Hao-wei Fu (3)
  Jian-zhong Huang (1)
  Angharad Gatehouse (2)
  Qing-yao Shu (1)
  
  1. State Key Laboratory of Rice Biology ; Institute of Crop Sciences ; Zhejiang University ; Hangzhou ; 310029 ; China
  2. Newcastle Institute for Research on Environment ; School of Biology ; University of Newcastle ; Newcastle upon Tyne ; NE1 7RU ; UK
  3. Jiaxing Academy of Agricultural Sciences ; Jiaxing ; 314016 ; China
  
• 关键词：CYP81A6 ; Bentazon ; Metsulfuron ; methyl ; Expression induction ; Xenobiotics ; S482.4 ; CYP81A6 ; 鑻揪鏉?/li> 鐢茬 ; 闅?/li> 璇卞琛ㄨ揪 ; 寮傛簮鐗?/li>
• 刊名：Journal of Zhejiang University SCIENCE B
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：16
• 期：2
• 页码：113-122
• 全文大小：1,675 KB
• 参考文献：1. Ahmad, P., Sarwat, M., Sharma, S., 2008. Reactive oxygen species, antioxidant and signaling in plants. / J. Plant Biol., lus-plus">51(3):167鈥?73. [doi:10.1007/BF03030694] CrossRef
  2. Courcelle, J., Khodursky, A., Hanawalt, P.C., 2001. Comparative gene expression profiles following UV exposure in wild-type and SOS-deficient / Escherichia coli. Genetics, lus-plus">158(1):41鈥?4.
  3. Deng, F., Hatzios, K.K., 2003. Characterization of cytochrome P450-mediated bensulfuron-methyl / O-demethylation in rice. / Pestic. Biochem. Physiol., lus-plus">74(2):102鈥?15. [doi:10.1016/S0048-3575(02)00151-7] CrossRef
  4. Dvorak, Z., Ulrichova, J., Modriansky, M., 2005. Role of microtubules network in / CYP genes expression. / Curr. Drug Metab., lus-plus">6(6):545鈥?52. [doi:10.2174/138920005774832623] CrossRef
  5. Edwards, R., Dixon, D.P., Cummins, I., / et al., 2011. New perspectives on the metabolism and detoxification of synthetic compounds in plants. / In: Schr枚der, P., Collins, C.D. (Eds.), Organic Xenobiotics and Plants, Vol 8. Springer Netherlands, p.125鈥?48. [doi:10.1007/978-90-481-9852-8_7] CrossRef
  6. Fu, H.W., 2004. Hybrid rice Jiayou 99 with fine quality and super-yield. / J. Anhui Agric. Sci., lus-plus">32(3):429鈥?32 (in Chinese).
  7. Gianessi, L.P., Reigner, N.P., 2007. The value of herbicides in US crop production. / Weed Technol., lus-plus">21(2):559鈥?66. [doi:10.1614/WT-06-130.1] CrossRef
  8. Glover, D.G., Schapaugh, W.T.Jr., 2002. Inheritance of resistance to pendimethalin herbicide induced stem damage in soybean. / Euphytica, lus-plus">125(3):433鈥?37. [doi:10.1023/A:1016078011794] CrossRef
  9. Green, J.M., 2009. Evolution of glyphosate-resistance crop technology. / Weed Sci., lus-plus">57(1):108鈥?17. [doi:10.1614/WS-08-030.1] CrossRef
  10. Han, Y.C., Wang, C.Y., 2002. Physiological basis of bentazon tolerance in rice ( / Oryza sativa L.) lines. / Weed Biol. Manag., lus-plus">2(4):186鈥?93. [doi:10.1046/j.1445-6664.2002.00067.x] CrossRef
  11. Hannemann, F., Bichet, A., Ewen, K.M., / et al., 2007. Cytochrome P450 systems鈥攂iological variations of electron transport chains. / Biochim. Biophys. Acta, lus-plus">1770(3):330鈥?44. [doi:10.1016/j.bbagen.2006.07.017] CrossRef
  12. Hiei, Y., Komari, T., 2008. / Agrobacterium-mediated transformation of rice using immature embryos or calli induced from mature seed. / Nat. Protoc., lus-plus">3(5):824鈥?34. [doi:10.1038/nprot.2008.46] CrossRef
  13. Konishi, T., Shinohara, K., Yamada, K., / et al., 1996. Acetyl-CoA carboxylase in higher plants: most plants other than Gramineae have both the prokaryotic and the eukaryotic forms of enzyme. / Plant Cell Physiol., lus-plus">37(2):117鈥?22. [doi:10.1093/oxfordjournals.pcp.a028920] CrossRef
  14. Lin, C.Y., Fang, J., Xu, X.L., / et al., 2008. A built-in strategy for containment of transgenic plants: creation of selectively terminable transgenic rice. / PLoS ONE, lus-plus">3(3):e1818. [doi:10.1371/journal.pone.0001818] CrossRef
  15. Liu, C., Liu, S.Q., Wang, F., / et al., 2012. Expression of a rice / CYP81A6 gene confers tolerance to bentazon and sulfonylurea herbicides in both / Arabidopsis and tobacco. / Plant Cell Tiss. Organ Cult., lus-plus">109(3):419鈥?28. [doi:10.1007/s11240-011-0106-5] CrossRef
  16. Liu, M.Y., Chen, W.W., Xu, J.M., / et al., 2013. The role of / VuMATE1 expression in aluminum-inducible citrate secretion in rice bean ( / Vigna umbellata) roots. / J. Exp. Bot., lus-plus">64(7):1795鈥?804. [doi:10.1093/jxb/ert039] CrossRef
  17. Livak, K.J., Schmittgen, T.D., 2001. Analysis of relative gene expression data using real-time quantitative PCR and the {ie121-1}. / Methods, lus-plus">25(4):402鈥?08. [doi:10.1006/meth.2001.1262] CrossRef
  18. Lv, M.Z., Chao, D.Y., Shan, J.X., / et al., 2012. Rice carotenoid 尾-ring hydroxylase CYP97A4 is involved in lutein biosynthesis. / Plant Cell Physiol., lus-plus">53(6):987鈥?002. [doi:10.1093/pcp/pcs041] CrossRef
  19. Narusaka, Y., Narusaka, M., Seki, M., / et al., 2004. Crosstalk in the responses to abiotic and biotic stress in arabidopsis: analysis of gene expression in cytochrome P450 gene superfamily by cDNA microarray. / Plant Mol. Biol., lus-plus">55(3): 327鈥?42. [doi:10.1007/s11103-004-0685-1] CrossRef
  20. Nelson, D.R., 2011. Progress in tracing the evolutionary paths of cytochrome P450. / Biochem. Biophys. Acta, lus-plus">1814(1): 14鈥?8. [doi:10.1016/j.bbapap.2010.08.008]
  21. Ohkawa, H., Tsujii, H., Ohkawa, Y., 1999. The use of cytochrome P450 genes to introduce herbicide tolerance in crops: a review. / Pestic. Sci., lus-plus">55(9):867鈥?74. [doi:10.1002/(SICI)1096-9063(199909)55:9銆?67::AID-PS31銆?.0.CO;2-S] CrossRef
  22. Omokawa, H., Wu, J.R., Hatzios, K.K., 1996. Mechanism of safening action of dymuron and its two monomethyl analogues against bensulfuron-methyl injury to rice ( / Oryza sativa). / Pestic. Biochem. Physiol., lus-plus">55(1):54鈥?3. [doi:10.1006/pest.1996.0035] CrossRef
  23. Pan, G., Zhang, X.Y., Liu, K.D., / et al., 2006. Map-based cloning of a novel rice cytochrome P450 gene / CYP81A6 that confers resistance to two different classes of herbicides. / Plant Mol. Biol., lus-plus">61(6):933鈥?43. [doi:10.1007/s11103-006-0058-z] CrossRef
  24. Pang, S.S., Guddat, L.W., Duggleby, R.G., 2003. Molecular basis of sulfonylurea herbicide inhibition of acetohydroxyacid synthase. / J. Biol. Chem., lus-plus">278(9):7639鈥?644. [doi:10.1074/jbc.M211648200] CrossRef
  25. Sterling, T.M., Balke, N.E., 1988. Use of soybean ( / Glycine max) and velvetleaf ( / Abutilon theopbrasti) suspension-cultured cells to study bentazon metabolism. / Weed Sci., lus-plus">36(5):558鈥?68.
  26. Wang, Q.Z., Fu, H.W., Huang, J.Z., / et al., 2012. Generation and characterization of bentazon susceptible mutants of commercial male sterile lines and evaluation of their utility in hybrid rice production. / Field Crop. Res., lus-plus">137: 12鈥?8. [doi:10.1016/j.fcr.2012.09.001] CrossRef
  27. Ward, E.R., Uknes, S.J., Williams, S.C., / et al., 1991. Coordinate gene activity in response to agents that induce systemic acquired resistance. / Plant Cell, lus-plus">3(10):1085鈥?094. [doi:10.1105/tpc.3.10.1085] CrossRef
  28. Yokosho, K., Yamaji, N., Ma, J.F., 2011. An Al-inducible MATE gene is involved in external detoxification of Al in rice. / Plant J., lus-plus">68(6):1061鈥?069. [doi:10.1111/j.1365-313X.2011.04757.x] CrossRef
  29. Yoo, S.D., Cho, Y.H., Sheen, J., 2007. / Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis. / Nat. Protoc., lus-plus">2(7):1565鈥?572. [doi:10.1038/nprot.2007.199] CrossRef
  30. Yuan, J.S., Tranel, P.J., Stewart, C.N.Jr., 2007. Non-target-site herbicide resistance: a family business. / Trends Plant Sci., lus-plus">12(1):6鈥?3. [doi:10.1016/j.tplants.2006.11.001] CrossRef
  31. Zhang, J., Xu, Y., Wu, X., / et al., 2002. A bentazon and sulfonylurea sensitive mutant: breeding, genetics, and potential application in seed production of hybrid rice. / Theor. Appl. Genet., lus-plus">105(1):16鈥?2. [doi:10.1007/s00122-002-0874-8] CrossRef
  32. Zhang, L., Lu, Q., Chen, H.G., / et al., 2006. Identification of a cytochrome P450 hydroxylase CYP81A6 as the candicate for the bentazon and sulfonylurea herbicide resistance gene, / Bel, in rice. / Mol. Breed., lus-plus">19(1):59鈥?8. [doi:10.1007/s11032-006-9044-z] CrossRef
  33. Zhao, Y.D., 2010. Auxin biosynthesis and its role in plant development. / Annu. Rev. Plant Biol., lus-plus">61(1):49鈥?4. [doi:10.1146/annurev-arplant-042809-112308] CrossRef
  
• 刊物主题：Biomedicine general;
• 出版者：Springer Berlin Heidelberg
• ISSN：1862-1783

文摘

The cytochrome P450 gene CYP81A6 confers tolerance to bentazon and metsulfuron-methyl, two selective herbicides widely used for weed control in rice and wheat fields. Knockout mutants of CYP81A6 are highly susceptible to both herbicides. The present study aimed to characterize the CYP81A6 expression in rice. Quantitative real-time polymerase chain reaction (PCR) analyses demonstrated that foliar treatment of bentazon (500 mg/L) greatly induced expression of CYP81A6 in both wild-type (Jiazhe B) and its knockout mutant (Jiazhe mB): a 10-fold increase at 9 h before returning to basal levels at 24 h in Jiazhe B, while in the mutant the expression level rose to >20-fold at 12 h and maintained at such high level up to 24 h post exposure. In contrast, metsulfuron-methyl (500 mg/L) treatment did not affect the expression of CYP81A6 in Jiazhe B within 80 h; thereafter the expression peaked at 120 h and returned gradually to basal levels by Day 6. We suggest that a metabolite of metsulfuron-methyl, 1H-2,3-benzothiazin-4-(3H)-one-2,2-dioxide, is likely to be responsible for inducing CYP81A6 expression, rather than the metsulfuron-methyl itself. Use of a promoter-GUS reporter construct (CYP81A6Pro::GUS) demonstrated that CYP81A6 was constitutively expressed throughout the plant, with the highest expression in the upper surfaces of leaves. Subcellular localization studies in rice protoplasts showed that CYP81A6 was localized in the endoplasmic reticulum. These observations advance our understanding of CYP81A6 expression in rice, particularly its response to the two herbicides.