High-throughput sequencing of methylated cytosine enriched by modification-dependent restriction endonuclease MspJI
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- 作者：Xiaojun Huang (1) (2)
Hanlin Lu (1)
Jun-Wen Wang (1)
Liqin Xu (1) (3)
Siyang Liu (1)
Jihua Sun (1)
Fei Gao (1) - 关键词：MspJI ; DNA methylation ; Arabidopsis
- 刊名：BMC Genetics
- 出版年：2013
- 出版时间：December 2013
- 年：2013
- 卷：14
- 期：1
- 全文大小：692KB
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Hanlin Lu (1)
Jun-Wen Wang (1)
Liqin Xu (1) (3)
Siyang Liu (1)
Jihua Sun (1)
Fei Gao (1)
1. Science & Technology Department, BGI-Shenzhen, Building No. 11, Bei Shan Industrial Zone, Yantian District, Shenzhen, 518083, China
2. College of Life Sciences, Wuhan University, Luojia Road No.16, Wuchang District, Wuhan, 430072, China
3. School of Life Science and Technology, University of Electronic Science and Technology of China, No.4, Section 2, North Jianshe Road, Chengdu, 610054, China
文摘
Background As a well-known epigenomic modification, DNA methylation is found to be common in plants and plays an important role in many biological processes. Relying on the unique feature of methylation-dependent digestion, the family of methylation-requiring restriction-like endonuclease, such as MspJI and its homologs, was suggested for a potential usage in methylation detection. Results In this study, we combine MspJI digestion and electrophoretic band selection with next generation high-throughput sequencing technology to detect 5-methylcytosines in Arabidopsis genome. By developing a bioinformatics workflow to attribute the CNNR sites recognized by MspJI to the reference genome, we fulfilled the systematic assessment of this method. Conclusions According to the assessment, here we provide the method for generating a detailed map of plant methylome that could be feasible, reliable and economical in methylation investigation.