The impact on secreted VEGF of Hep-2 human laryngeal cancer cell induced by Rg3

详细信息    查看全文

• 作者：Jihua Zhang (1)
  Lai Wang (2)
  Caili Han (1)
  Dongmei Song (1)
  Baoshan Wang (3)
  Suqin Shi (2)
  Sha Liu (2)
  
• 关键词：Rg3 ; secreted VEGF ; Hep ; 2 cell ; HVEC
• 刊名：The Chinese-German Journal of Clinical Oncology
• 出版年：2013
• 出版时间：October 2013
• 年：2013
• 卷：12
• 期：10
• 页码：477-480
• 全文大小：
• 作者单位：Jihua Zhang (1)
  Lai Wang (2)
  Caili Han (1)
  Dongmei Song (1)
  Baoshan Wang (3)
  Suqin Shi (2)
  Sha Liu (2)
  
  1. Department of Otolaryngology-Head and Neck Surgery, The First Hospital of Hebei Medical University, Shijiazhuang, 050031, China
  2. Intensive Care Unit, the First Hospital of Hebei Medical University, Shijiazhuang, 050031, China
  3. Hebei Medical University, Shijiazhuang, 050031, China
  
• ISSN：1613-9089

文摘

Objective The aim of this study was to investigate the affecting of Rg3 to secreted VEGF of human laryngeal carcinoma Hep-2 cells and its mechanism of inhibition to tumor angiogenesis. Methods Cultured human laryngeal cancer cell line Hep-2 and human vascular endothelial cells in vitro, cells got into the period of exponential phase of growth, was diviced into 3 groups: group I (control group), group II (DDP group), group III (Rg3 group). Added to the Hep-2 cells Rg3 and DDP, made Rg3 final concentration was 300 μg/mL, and DDP was 3 μg/mL. 48 h later, specimens from sample to be done immunocytochemistry, and the protein of VEGF in Hep-2 cells to be detected. Collecting Hep-2 cells supernatant, some was used to measure the protein level of VEGF in Hep-2 cells supernatant by ELISA. Some was used to culture HVEC. 24 h later, cell growth inhibition rate of human vascular endothelial was determined by MTT. Results The protein level of VEGF was evidently higher in group I compared to group II and group III, it was not only in Hep-2 cells, but also in supernatant of Hep-2 cells. There was no significantly different between group II and group III. MTT results showed that, the human vascular endothelial cell growth inhibition rate of group I was significantly lower than that of group II and group III (P < 0.05). At the same time the HVEC growth inhibition rate of group II was significantly lower than that of group III (P < 0.05). Conclusion The inhibition to tumor angiogenesis of Rg3 is stronger than traditional chemotherapy drug cisplatin. It worke by reducing the biological effects of secreted VEGF, But the effecting worke by reducing the activity of secreted VEGF itself or affecting endothelial function of VEGF receptor or some other ways to be further studied.