Soil-Derived Microbial Consortia Enriched with Different Plant Biomass Reveal Distinct Players Acting in Lignocellulose Degradation
详细信息 查看全文
- 作者：Maria Julia de Lima Brossi ; Diego Javier Jiménez…
- 关键词：Plant biomass ; Bioconversion ; Bacterial–fungal consortia ; (Hemi) cellulolytic activity
- 刊名：Microbial Ecology
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：71
- 期：3
- 页码：616-627
- 全文大小：1,251 KB
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Microbiology
Ecology
Geoecology and Natural Processes
Nature Conservation - 出版者：Springer New York
- ISSN：1432-184X
文摘
Here, we investigated how different plant biomass, and—for one substrate—pH, drive the composition of degrader microbial consortia. We bred such consortia from forest soil, incubated along nine aerobic sequential - batch enrichments with wheat straw (WS1, pH 7.2; WS2, pH 9.0), switchgrass (SG, pH 7.2), and corn stover (CS, pH 7.2) as carbon sources. Lignocellulosic compounds (lignin, cellulose and xylan) were best degraded in treatment SG, followed by CS, WS1 and WS2. In terms of composition, the consortia became relatively stable after transfers 4 to 6, as evidenced by PCR-DGGE profiles obtained from each consortium DNA. The final consortia differed by ~40 % (bacteria) and ~60 % (fungi) across treatments. A ‘core’ community represented by 5/16 (bacteria) and 3/14 (fungi) bands was discerned, next to a variable part. The composition of the final microbial consortia was strongly driven by the substrate, as taxonomically-diverse consortia appeared in the different substrate treatments, but not in the (WS) different pH one. Biodegradative strains affiliated to Sphingobacterium kitahiroshimense, Raoultella terrigena, Pseudomonas putida, Stenotrophomonas rhizophila (bacteria), Coniochaeta ligniaria and Acremonium sp. (fungi) were recovered in at least three treatments, whereas strains affiliated to Delftia tsuruhatensis, Paenibacillus xylanexedens, Sanguibacter inulus and Comamonas jiangduensis were treatment-specific.