Systematic assessment of reference genes for RT-qPCR across plant species under salt stress and drought stress

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• 作者：Xinlong Xiao ; Xiaomeng Wu ; Jinbiao Ma ; Pengbo Li ; Taotao Li…
• 关键词：Real ; time quantitative PCR ; Normalization ; Multi ; gene family ; geNorm ranking ; Abiotic stress ; Meta ; analysis
• 刊名：Acta Physiologiae Plantarum
• 出版年：2015
• 出版时间：September 2015
• 年：2015
• 卷：37
• 期：9
• 全文大小：775 KB
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• 作者单位：Xinlong Xiao (1) (2)
  Xiaomeng Wu (1) (2)
  Jinbiao Ma (1)
  Pengbo Li (1) (2)
  Taotao Li (1) (2)
  Yinan Yao (1)
  
  1. Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Science, Ürümqi, China
  2. University of Chinese Academy of Sciences, Beijing, China
  
• 刊物主题：Plant Physiology; Plant Genetics & Genomics; Plant Biochemistry; Plant Pathology; Plant Anatomy/Development; Agriculture;
• 出版者：Springer Berlin Heidelberg
• ISSN：1861-1664

文摘

Suitable reference gene (RGs) is the prerequisite for accurate normalization of real-time quantitative PCR (RT-qPCR) data. However, previous results are diverse in various researches that focused on selecting stable RGs. This study aims at systematically assessing various RGs in plants under salt stress or drought stress by collection of geNorm rankings of genes, data transformation and statistic analysis. Although none of the analyzed genes can guarantee universally stable expressions in plant species under salt stress or drought stress, we found that 18S (18S ribosomal RNA) was generally the least stable gene under salt and drought stress. This gene should not be used as the RG in RT-qPCR. On the contrary, it is least risk to use EF1 for salt stress and TIP41 for drought treatment experiments. We compared the effects of salt and drought stresses on 7 frequently used RGs through paired-samples T test. The expression of Ubiquitin gene under drought stress is much more unstable than that under salt stress. The tested genes belonging to multi-gene family and having different stability could be one reason of variations in the published studies, which was supported by the analysis of expression profile of Salicornia europaea transcriptome. This is the first systematic assessment quantifying global stability of RGs across plant species under salt stress and drought stress, which will improve our understanding of RGs and facilitate the future work on RGs selection.