Purification, characterization and procoagulant activity of polysaccharides from Angelica dahurice roots

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• 作者：Jinmei Wang ; Pengli Lian ; Qi Yu ; Jinfeng Wei ; Wen-yi Kang
• 关键词：Angelicae dahuricae Radix ; Polysaccharides ; Procoagulant
• 刊名：Chemistry Central Journal
• 出版年：2017
• 出版时间：December 2017
• 年：2017
• 卷：11
• 期：1
• 全文大小：2773KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry/Food Science, general;
• 出版者：Springer International Publishing
• ISSN：1752-153X
• 卷排序：11

文摘

Five polysaccharides, namely ADPs-1a, ADPs-1b, ADPs-2, ADPs-3a and ADPs-3b, were extracted from Angelicae dahuricae Radix, purified, and identified by high performance gel permeation chromatography (HPSEC), gas chromatography (GC), Fourier transform infrared (FT-IR) spectrometer and nuclear magnetic resonance spectra (NMR), including the determination of procoagulant activity in vitro. The average molecular weight (Mw) of the polysaccharides was 153,800, 8312, 111,700, 3766 and 96,680 g/mol, respectively. Coagulation assays indicated that ADPs-1b, ADPs-2, ADPs-3a and ADPs-3b had procoagulant activities. ADPs-1b exerted the procoagulant activities through intrinsic pathway, extrinsic pathway and increased the content of FIB in vitro. ADPs-2 exerted the procoagulant activities through intrinsic pathway and extrinsic pathway. ADPs-3a had procoagulant activities and the activity was associated with the intrinsic pathway and increased the content of FIB. ADPs-3b exerted the activities through extrinsic pathway and increased the content of FIB.