A novel dual-targeted ultrasound contrast agent provides improvement of gene delivery efficiency in vitro

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• 作者：Jinfeng Xu ; Xinxin Zeng ; Yingying Liu ; Hui Luo ; Zhanghong Wei ; Huiyu Liu…
• 刊名：Tumor Biology
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：37
• 期：7
• 页码：8609-8619
• 全文大小：3,188 KB
• 刊物主题：Cancer Research;
• 出版者：Springer Netherlands
• ISSN：1423-0380
• 卷排序：37

文摘

Ultrasound-targeted microbubble destruction (UTMD) has become a novel gene/drug delivery method in cancer therapeutic application. However, the gene transfection efficiency mediated by UTMD is still unsatisfactory. Here, we introduced iRGD/CCR2 dual-targeted cationic microbubbles (MB_iRGD/CCR2) which was modified with PEI-600 and coated with iRGD peptides and anti-CCR-2 antibodies. It showed that MB_iRGD/CCR2 had a 25.83 ± 1.57 mV surface zeta potential and good stability. The experiments in vitro showed MB_iRGD/CCR2 had higher binding efficiency with both bEnd.3 cells and MCF-7 cells than that of iRGD or CCR2 single-targeted cationic microbubbles (MB_iRGD or MB_CCR2) (P < 0.05 for both). Agarose gel electrophoresis assay showed that MB_iRGD/CCR2 can effectively load pGPU6/GFP/Neo-shAKT2 plasmid DNA. Compared with the plain MBs (MB_control) or single-targeted cationic MBs including MB_iRGD and MB_CCR2 (P < 0.05 for all), the dual-targeted cationic MB_iRGD/CCR2 groups had higher gene transfection efficiency under US exposure. It showed that the dual-targeted cationic MB_iRGD/CCR2 has a potential value to be used as an ultrasound imaging probe for ultrasound image-guided tumor gene therapy.KeywordsUltrasoundMicrobubblesDual-targetedGene delivery