Platycodin D Attenuates Airway Inflammation in a Mouse Model of Allergic Asthma by Regulation NF-κB Pathway

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• 作者：Tianzhu Zhang ; Shihai Yang ; Juan Du ; Yao Jinfu ; Wang Shumin
• 关键词：platycodin D ; asthma ; NF ; κB pathway
• 刊名：Inflammation
• 出版年：2015
• 出版时间：June 2015
• 年：2015
• 卷：38
• 期：3
• 页码：1221-1228
• 全文大小：1,112 KB
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• 作者单位：Tianzhu Zhang (1)
  Shihai Yang (2)
  Juan Du (3)
  Yao Jinfu (1)
  Wang Shumin (1)
  
  1. Changchun University of Chinese Medicine, Changhcun, 130117, China
  2. Jilin Agricultural University, Changchun, 130118, China
  3. School of Life Science, Peking University, Beijing, 100871, China
  
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Rheumatology
  Internal Medicine
  Pharmacology and Toxicology
  Pathology
  
• 出版者：Springer Netherlands
• ISSN：1573-2576

文摘

In this work, the anti-asthma potential of platycodin D (PLD) was studied by investigation of its effect to suppress airway inflammation, a murine model of asthma and the possible mechanisms. A total of 50 mice were randomly assigned to five experimental groups: control, ovalbumin (OVA), OVA+ dexamethasone (2?mg/kg) and OVA-?PLD (40, 80?mg/kg). Airway resistance (Raw) were measured; airway histological studies were evaluated by the hematoxylin and eosin (HE) staining; interleukin-4 (IL-4), interleukin-5(IL-5), and interleukin-13 in bronchoalveolar lavage fluid (BALF) were evaluated by enzyme-linked immunosorbent assay (ELISA); NF-κBp65, p-NF-κBp65, p-IKKα, IKKα, p-IKKβ, p-IкBα, and IкBα of airway were measured by Western blotting. Our study demonstrated that PLD inhibited OVA-induced increases in Raw and eosinophil count in airway; IL-4, IL-5, and IL-13 were recovered in BALF. Histological studies demonstrated that PLD substantially inhibited OVA-induced eosinophilia in airway tissue. Western blotting studies demonstrated that PLD substantially inhibited NF-κB pathway. These findings suggest that PLD may effectively ameliorate the progression of asthma and could be used as a therapy for patients with allergic asthma.