Melatonin Treatment Protects Against Acute Spinal Cord Injury-Induced Disruption of Blood Spinal Cord Barrier in Mice
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- 作者：Qingbin Wu (1)
Yingli Jing (1)
Xiaochen Yuan (1)
Xiaoyan Zhang (1)
Bingwei Li (1)
Mingming Liu (1)
Bing Wang (1)
Hongwei Li (1)
Shuying Liu (1)
Ruijuan Xiu (1) - 关键词：Spinal cord injury ; Microcirculation ; Blood spinal cord barrier ; Permeability ; Melatonin
- 刊名：Journal of Molecular Neuroscience
- 出版年：2014
- 出版时间：December 2014
- 年：2014
- 卷：54
- 期：4
- 页码：714-722
- 全文大小：2,433 KB
- 参考文献：1. Allen A (1911) Surgery of experimental lesion of spinal cord equivalent to crush injury of fracture dislocation of spinal column. A preliminary report. JAMA 57:878-80 CrossRef
2. Allen A (1914) Remarks on the histopathological changes in the spinal cord due to impact. An experiment study. J Nerv Ment Dis 41:141-147 CrossRef
3. Alvarez C, Alonso-Muriel I, Garcia G, Crespo J, Bellver J, Simon C, Pellicer A (2007) Implantation is apparently unaffected by the dopamine agonist cabergoline when administered to prevent ovarian hyperstimulation syndrome in women undergoing assisted reproduction treatment: a pilot study. Hum Reprod 22:3210-214 CrossRef
4. Casella GT, Bunge MB, Wood PM (2006) Endothelial cell loss is not a major cause of neuronal and glial cell death following contusion injury of the spinal cord. Exp Neurol 202:8-0 CrossRef
5. Chen HY, Chen TY, Lee MY, Chen ST, Hsu YS, Kuo YL, Chang GL, Wu TS, Lee EJ (2006) Melatonin decreases neurovascular oxidative/nitrosative damage and protects against early increases in the blood–brain barrier permeability after transient focal cerebral ischemia in mice. J Pineal Res 41:175-82 CrossRef
6. Daneman R, Zhou L, Kebede AA, Barres BA (2010) Pericytes are required for blood–brain barrier integrity during embryogenesis. Nature 468:562-66 CrossRef
7. Dulmovits BM, Herman IM (2012) Microvascular remodeling and wound healing: a role for pericytes. Int J Biochem Cell Biol 44:1800-812 CrossRef
8. Echeverry S, Shi XQ, Rivest S, Zhang J (2011) Peripheral nerve injury alters blood-spinal cord barrier functional and molecular integrity through a selective inflammatory pathway. J Neurosci 31:10819-0828 CrossRef
9. Ersahin M, Toklu HZ, Cetinel S, Yuksel M, Yegen BC, Sener G (2009) Melatonin reduces experimental subarachnoid hemorrhage-induced oxidative brain damage and neurological symptoms. J Pineal Res 46:324-32 CrossRef
10. Espino J, Bejarano I, Ortiz A, Lozano GM, Garcia JF, Pariente JA, Rodriguez AB (2010) Melatonin as a potential tool against oxidative damage and apoptosis in ejaculated human spermatozoa. Fertil Steril 94:1915-917 CrossRef
11. Esposito E, Genovese T, Caminiti R, Bramanti P, Meli R, Cuzzocrea S (2008) Melatonin regulates matrix metalloproteinases after traumatic experimental spinal cord injury. J Pineal Res 45:149-56 CrossRef
12. Fan ZK, Cao Y, Lv G, Wang YS, Guo ZP (2013a) The effect of cigarette smoke exposure on spinal cord injury in rats. J Neurotrauma 30:473-79 CrossRef
13. Fan ZK, Lv G, Wang YF, Li G, Yu DS, Wang YS, Zhang YQ, Mei XF, Cao Y (2013b) The protective effect of salvianolic acid B on blood-spinal cord barrier after compression spinal cord injury in rats. J Mol Neurosci 51:986-93 CrossRef
14. Furuse M, Tsukita S (2006) Claudins in occluding junctions of humans and flies. Trends Cell Biol 16:181-88 CrossRef
15. Goldberg AL, Kershah SM (2010) Advances in imaging of vertebral and spinal cord injury. J Spinal Cord Med 33:105-16
16. Griffiths IR, Burns N, Crawford AR (1978) Early vascular changes in the spinal grey matter following impact injury. Acta Neuropathol 41:33-9- 作者单位：Qingbin Wu (1)
Yingli Jing (1)
Xiaochen Yuan (1)
Xiaoyan Zhang (1)
Bingwei Li (1)
Mingming Liu (1)
Bing Wang (1)
Hongwei Li (1)
Shuying Liu (1)
Ruijuan Xiu (1)
1. Key Laboratory for Microcirculation, Ministry of National Health of China, Institute of Microcirculation, Chinese Academy of Medical Sciences and Peking Union Medical College, 5 Dong Dan San Tiao, 100005, Beijing, China- ISSN：1559-1166
- 作者单位：Qingbin Wu (1)
文摘
The spinal cord microcirculation plays a critically important role in maintaining the normal function of spinal cord neurons, glial cells, and axons. Previous researches were largely focused on improved neurological manifestations of spinal cord injury (SCI) while ignoring to improve spinal cord microcirculation disorder after melatonin treatment. Therefore, the mechanism of melatonin that affects blood spinal cord barrier (BSCB) integrity and microcirculation in SCI remains unclear. The present study was performed to investigate the effect of melatonin on the BSCB in a SCI mice model. Melatonin (5, 10, 25, 50, 100?mg/kg i.p.) was administered to mice immediately following SCI. Compared to the 48?h post-SCI group, mice treated with melatonin (50?mg/kg) exhibited significantly reduced BSCB permeability. Additionally, melatonin treatment restrained microvessel loss; attenuated edema; protected the tight junction proteins, endothelial cells, and pericytes; decreased the number of cell apoptosis; and reduced MMP3/AQP4/HIF-1α/VEGF/VEGFR2 expression after SCI. Above all, our results clearly demonstrated that melatonin could stabilize microvascular barrier function and microcirculation of SCI, whose mechanism was to promote the repair of the damaged BSCB.