Polygala tenuifolia polysaccharide (PTP) inhibits cell proliferation by repressing Bmi-1 expression and downregulating telomerase activity

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• 作者：Fubin Zhang ; Xiaowei Song ; Li Li ; Jingfang Wang ; Leyuan Lin ; Cong Li…
• 关键词：Polygala tenuifolia ; Polysaccharide ; Human ovarian cancer ; Bmi ; 1 ; Telomerase ; Antitumor
• 刊名：Tumor Biology
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：36
• 期：4
• 页码：2907-2912
• 全文大小：409 KB
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• 作者单位：Fubin Zhang (1)
  Xiaowei Song (2)
  Li Li (2)
  Jingfang Wang (2)
  Leyuan Lin (3)
  Cong Li (4)
  Hongtao Li (5)
  Yanju Lv (2)
  Yinghua Jin (2)
  Ying Liu (2)
  Yu Hu (2)
  Tao Xin (2)
  
  1. Department of Gynecology, The Third Affiliated (Tumor) Hospital, Harbin Medical University, Harbin, 150040, China
  2. Department of Medical Oncology, The Second Affiliated Hospital, Harbin Medical University, Harbin, 150081, China
  3. The 209 Hospital of People’s Liberation Army (PLA), Mudanjiang, 157000, China
  4. Department of Pathology, The Third Affiliated (Tumour) Hospital, Harbin Medical University, Harbin, 150040, China
  5. Department of Molecular Medicine, Mayo Clinic, Rochester, MN, 55905, USA
  
• 刊物主题：Cancer Research;
• 出版者：Springer Netherlands
• ISSN：1423-0380

文摘

In our previous study, we isolated a homogeneous polysaccharide (PTP) with antitumor activity from the roots of Polygala tenuifolia. In view of the close correlation between Bmi-1 expression and progression of ovarian cancer, we intend to elucidate the mechanism of its activity by determining the Bmi-1 expression and the telomerase activity in human ovarian carcinoma OVCAR-3 cells following treatment with PTP at three concentrations of 0.5, 1, and 2?mg/mL for 48?h. MTT and colony-forming assays revealed that PTP had a significant inhibitory effect on the cell growth and colony formation of OVCAR-3 cells. Furthermore, Western blot and real-time PCR analysis showed that PTP inhibited Bmi-1 both in protein and transcript levels. Besides, the telomerase activity in OVCAR-3 cells was also downregulated after PTP treatment for 48?h. Taken together, the inhibitory effect of PTP on the cell growth was at least in part mediated via the downregulation of Bmi-1 expression and the telomerase activity in OVCAR-3 cells, and PTP might be a new candidate for chemotherapeutic agent against human ovarian cancer.