Heterologous expression of Arabidopsis C-repeat binding factor 3 (AtCBF3) and cold-regulated 15A (
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- 作者：Faxiang Wan (1)
Yu Pan (1)
Jinghua Li (1)
Xiangfu Chen (1)
Yanglu Pan (1)
Yongqing Wang (2)
Shibing Tian (2)
Xingguo Zhang (1) - 关键词：Eggplant ; AtCBF3 ; AtCOR15A ; Transgene ; Chilling tolerance
- 刊名：Plant Cell Reports
- 出版年：2014
- 出版时间：December 2014
- 年：2014
- 卷：33
- 期：12
- 页码：1951-1961
- 全文大小：2,692 KB
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Jinghua Li (1)
Xiangfu Chen (1)
Yanglu Pan (1)
Yongqing Wang (2)
Shibing Tian (2)
Xingguo Zhang (1)
1. Key Laboratory of Horticulture Science for Southern Mountainous Regions (Chinese Ministry of Education), College of Horticulture and Landscape Architecture, Southwest University, Chongqing, 400715, People’s Republic of China
2. Institute of Vegetable and Flower Research, Chongqing Academy of Agricultural Sciences, Chongqing, 401329, People’s Republic of China - ISSN：1432-203X
文摘
Key message Our study shows that the expression of AtCBF3 and AtCOR15A improved the chilling tolerance in transgenic eggplant. Abstract In an attempt to improve chilling tolerance of eggplant (Solanum melongena L) plants, Arabidopsis C-repeat binding factor 3 (AtCBF3) and cold-regulated 15A (AtCOR15A) genes both driven by an Arabidopsis RESPONSIVE TO DESSICATION 29A promoter (AtRD29A) were transferred into the plants of eggplant cultivar Sanyueqie. Two independent homozygous transgenic lines were tested for their cold tolerance. The leaves of the transgenic plants in both lines withered much slower and slighter than the wild-type plants after exposure to cold stress treatment at 2?±?1?°C. The gene expression of AtCBF3 and AtCOR15A was significantly increased as well as the proline content and the levels of catalase and peroxidase activities, while the relative electrical conductivity and the malondialdehyde content were remarkably decreased in the transgenic plants compared with the wild type at 4?±?0.5?°C. The results showed that the expression of the exogenous AtCBF3 and AtCOR15A could promote the cold adaptation process to protect eggplant plants from chilling stress.