A High Yield Method of Extracting Alkaloid from Aconitum coreanum by Pulsed Electric Field

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• 作者：Yongzhi Bai (1)
  Changyu Li (1)
  Jinghui Zhao (1)
  Peihe Zheng (1)
  Yali Li (1)
  Yongqi Pan (2)
  Yingping Wang (1)
  
• 关键词：LC–ESI–MS ; Pulsed electric field ; Aconitum coreanum ; Guanfu base A
• 刊名：Chromatographia
• 出版年：2013
• 出版时间：12 - June 2013
• 年：2013
• 卷：76
• 期：11
• 页码：635-642
• 全文大小：422KB
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• 作者单位：Yongzhi Bai (1)
  Changyu Li (1)
  Jinghui Zhao (1)
  Peihe Zheng (1)
  Yali Li (1)
  Yongqi Pan (2)
  Yingping Wang (1)
  
  1. Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, Jilin, 130122, China
  2. Huadi Bio-technology Development Co., Ltd., Jilin, 130044, China
  
• ISSN：1612-1112

文摘

A high yield method to extract alkaloids from Aconitum coreanum using pulsed electric field (PEF) was developed, and the optimized extraction method compared to the other four extraction methods, consisting of cold maceration extraction, percolation extraction, heat reflux extraction (HRE), and ultrasonic-assisted extraction (UE). The experimental factors of the extraction methods such as electric field intensity, pulse frequency and solid-to-solvent ratio were evaluated. The content of Guanfu base A (GFA) was quantified by liquid chromatography coupled with electrospray ionization tandem mass spectrometry. The results indicated that the highest yield of GFA was 3.94?mg?g? by PEF with conditions of 20?kV?cm? electric field intensity, 8 pulse, 1:12 solid-to-solvent ratio, and 90?% ethanol–water solution. Meanwhile, the extraction time of PEF was <1?min, which is much less than the HRE of 10?h and even the newly used technique UE of 40?min. Moreover, the results of PEF extraction method showed obvious advantages, with the highest efficiency (120?L?h?), the shortest extraction time (0.5-?min), and the lowest energy costs, which could be applied in the industrial production of alkaloids from A. coreanum. Therefore, the application of the PEF extraction method is a promising and constructive method for extraction of GFA.