Difference in apoptosis-associated genes expression profiling and immunohistology analysis between Kashin-Beck disease and primary osteoarthritis

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• 作者：Shixun Wu (1) (2)
  Chen Duan (1)
  Feng Zhang (1)
  Robert Pierce McKenzie (3)
  Jingjing Zheng (1)
  Umer Farooq (4)
  Yidong Bai (3)
  Xiong Guo (1)
  
• 关键词：Microarray ; Ingenuity Pathway Analysis ; Immunohistochemistry ; Kashin ; Beck disease ; Apoptosis
• 刊名：Chinese Science Bulletin
• 出版年：2014
• 出版时间：March 2014
• 年：2014
• 卷：59
• 期：9
• 页码：833-839
• 全文大小：819 KB
• 参考文献：1. Statistical bulletin of the development circumstance of Chinese Health Service in 2012 (2013) China. http://www.moh.gov.cn/mohwsbwstjxxzx/s7967/201306/fe0b764da4f74b858eb55264572eab92.shtml. Accessed 1 Oct 2013
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• 作者单位：Shixun Wu (1) (2)
  Chen Duan (1)
  Feng Zhang (1)
  Robert Pierce McKenzie (3)
  Jingjing Zheng (1)
  Umer Farooq (4)
  Yidong Bai (3)
  Xiong Guo (1)
  
  1. School of Public Health, College of Medicine, Key Laboratory of Environment and Gene Related Diseases of Ministry Education, Key Laboratory of Trace Elements and Endemic Diseases, Ministry of Health, Xi’an Jiaotong University, Xi’an, 710061, China
  2. Department of Orthopedics Surgery, The First Affiliated Hospital, College of Medicine, Xi’an Jiaotong University, Xi’an, 710061, China
  3. Department of Cellular and Structural Biology, University of Texas Health Sciences Center at San Antonio, 7703 Floyd Curl, San Antonio, TX, 78229, USA
  4. Department of Community Medicine, Ayub Medical College, Abbottabad, Pakistan
  
• ISSN：1861-9541

文摘

Kashin-Beck disease (KBD) is a chronic and deformed endemic osteoarthritis, without fully known etiology. As compared to primary osteoarthritis (POA), its pathogenesis still exists controversial. We performed this study to discriminate the difference in genes expression involved in apoptosis from KBD cartilages and POA cartilages. Microarray analysis and Ingenuity Pathway Analysis (IPA) were used to identify the molecular mechanisms/canonical pathways implicated in KBD arthritis. Immunohistochemistry staining was carried out to detect tissue distribution of PDCD5 and EGR1 in the knee cartilages from KBD and POA. The 23 up-regulated apoptosis-related genes with >2-fold change were identified. “Role of Macrophages, Fibroblasts, and Endothelial Cells in Rheumatoid Arthritis-signaling was screened as the most relevant pathway through IPA. The 8 differentially regulated genes were verified by qRT-PCR analysis. Programmed cell death 5 in middle and deeper zones and early growth response 1 in superficial and middle zones showed protein overexpressed in KBD cartilage samples comparing to those in POA cartilage samples. Data indicates a higher expression level of apoptosis-related functional genes in KBD articular cartilage compared with POA articular cartilage.