Production of cyclic adenosine-3鈥?5鈥?monophosphate by whole cell catalysis using recombinant Escherichia coli overexpressing adenylate cyclase

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• 作者：Nan Li (1202)
  Ying He (1202)
  Yong Chen (1202)
  Xiaochun Chen (1202)
  Jianxin Bai (1202)
  Jinglan Wu (1202)
  Jingjing Xie (1202)
  Hanjie Ying (1202)
  
• 关键词：cAMP ; Adenylate Cyclase ; Whole Cell Catalysis ; Bioconversion
• 刊名：Korean Journal of Chemical Engineering
• 出版年：2013
• 出版时间：April 2013
• 年：2013
• 卷：30
• 期：4
• 页码：913-917
• 全文大小：579KB
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• 作者单位：Nan Li (1202)
  Ying He (1202)
  Yong Chen (1202)
  Xiaochun Chen (1202)
  Jianxin Bai (1202)
  Jinglan Wu (1202)
  Jingjing Xie (1202)
  Hanjie Ying (1202)
  
  1202. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, 210009, P. R. China
  
• ISSN：1975-7220

文摘

Adenylate cyclase (EC 4.6.1.1) catalyzes the formation of cyclic adenosine-3鈥?5鈥?monophosphate (cAMP) from adenosine 5鈥?triphosphate (ATP). Recombinant Escherichia coli overexpressing adenylate cyclase was used to synthesize cAMP by whole cell catalysis. Some key parameters were examined during the catalytic process, while pH and Mg2+ were found to influence cAMP production significantly. Optimum conditions were pH 8.52 and 30 掳C with 77.2 mM Mg2+ in 100 mM Tris-HCl buffer, including 0.25% Triton-X 100 as detergent and 30 mM pyruvate sodium as enzyme activator for 6 h. 14.93 g/L of cAMP was produced with a conversion rate of 91.5%. The current work provided a potential way for the industrial production of cAMP.