Production of cyclic adenosine-3-5-monophosphate by whole cell catalysis using recombinant Escherichia coli overexpressing adenylate cyclase

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• 作者：Nan Li (1202)
  Ying He (1202)
  Yong Chen (1202)
  Xiaochun Chen (1202)
  Jianxin Bai (1202)
  Jinglan Wu (1202)
  Jingjing Xie (1202)
  Hanjie Ying (1202)
  
• 关键词：cAMP ; Adenylate Cyclase ; Whole Cell Catalysis ; Bioconversion
• 刊名：Korean Journal of Chemical Engineering
• 出版年：2013
• 出版时间：April 2013
• 年：2013
• 卷：30
• 期：4
• 页码：913-917
• 全文大小：579KB
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• 作者单位：Nan Li (1202)
  Ying He (1202)
  Yong Chen (1202)
  Xiaochun Chen (1202)
  Jianxin Bai (1202)
  Jinglan Wu (1202)
  Jingjing Xie (1202)
  Hanjie Ying (1202)
  
  1202. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, 210009, P. R. China
  
• ISSN：1975-7220

文摘

Adenylate cyclase (EC 4.6.1.1) catalyzes the formation of cyclic adenosine-3-5-monophosphate (cAMP) from adenosine 5-triphosphate (ATP). Recombinant Escherichia coli overexpressing adenylate cyclase was used to synthesize cAMP by whole cell catalysis. Some key parameters were examined during the catalytic process, while pH and Mg2+ were found to influence cAMP production significantly. Optimum conditions were pH 8.52 and 30 °C with 77.2 mM Mg2+ in 100 mM Tris-HCl buffer, including 0.25% Triton-X 100 as detergent and 30 mM pyruvate sodium as enzyme activator for 6 h. 14.93 g/L of cAMP was produced with a conversion rate of 91.5%. The current work provided a potential way for the industrial production of cAMP.