Rapid generation of dendritic cell specific transgenic mice by lentiviral vectors

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• 作者：Jinyu Zhang ; Liyun Zou ; Qin Liu ; Jingyi Li ; Jingran Zhou ; Yong Wang ; Na Li ; Ting Liu ; Hong Wei and Min Wu ; et al.
• 关键词：Dendritic cells ; CD11c ; Lentiviral vectors ; Transgenic mice
• 刊名：Transgenic Research
• 出版年：2009
• 出版时间：December, 2009
• 年：2009
• 卷：18
• 期：6
• 页码：921-931
• 全文大小：467.3 KB

文摘

Dendritic cell (DC) specific transgenic mice are a most important model for investigating dendritic cell functions in vivo. Recently, lentivirus mediated gene transfer has become a powerful and convenient method for generation of transgenic mice. We cloned a 1.2 kb CD11c promoter and constructed a lentiviral vector, which efficiently drove DC-specific expression in vitro. After microinjection of purified virus into the perivitelline space of single-cell embryo, more than 80 % newborn mice were transgenic and 7 F0 founders were rapidly generated in 2 months. GFP was strictly expressed in CD11c+ cells in spleens, thymus and lymph nodes of the transgenic mice. Importantly, the physiological characteristics and functions of DCs in the transgenic mice were not altered by the specific expression. These results indicate that this vector could be used to rapidly prepare DC-specific transgenic mice. Thus, this lentiviral vector system may provide a convenient and useful tool to study the properties of DCs in vivo.