Effect of salinity on physiological characteristics, yield and quality of microtubers in vitro in potato

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• 作者：Zhijun Zhang (1) (2)
  Bizeng Mao (1)
  Huizhen Li (2)
  Weijun Zhou (1)
  Yasutomo Takeuchi (3)
  Koichi Yoneyama (3)
  
• 关键词：Carbohydrate ; malondialdehyde (MDA) ; microtuber ; potato (Solanum tuberosum L.) ; proline ; salinity ; starch ; sugars ; vitamin C (Vc)
• 刊名：Acta Physiologiae Plantarum
• 出版年：2005
• 出版时间：December 2005
• 年：2005
• 卷：27
• 期：4
• 页码：481-489
• 全文大小：195KB
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• 作者单位：Zhijun Zhang (1) (2)
  Bizeng Mao (1)
  Huizhen Li (2)
  Weijun Zhou (1)
  Yasutomo Takeuchi (3)
  Koichi Yoneyama (3)
  
  1. College of Agriculture and Biotechnology, Zhejiang University, 310029, Hangzhou, China
  2. Department of Environment and Safety Engineering, North University of China, 030051, Taiyuan, China
  3. Center for Research on Wild Plants, Utsunomiya University, 321-8505, Utsunomiya, Japan
  

文摘

In vitro microtuberization provides an adequate experimental model for the physiological and metabolic studies of tuberization and the preliminary screenings of potential potato genotypes. The effects of saline stress at 0-0 mmol concentration on in vitro tuberization of two potato cultivars were investigated in this study. With an increase in the salt concentration, the microtuberization of potato was either delayed by 5-0 days (20 and 40 mmol NaCl) or inhibited completely (80 mmol NaCl) in addition to the reduction in microtuber yields. The two potato genotypes studied showed different trends in total soluble sugars, sucrose and starch contents of microtubers under NaCl stress, while glucose and fructose levels remained unchanged. The vitamin C content in microtubers of two potato genotypes was reduced by salt stress. Salinity applied from 20 to 60 mmol progressively increased proline and malondialdehyde (MDA) levels in microtubers of both the potato cultivars. In genotype Zihuabai, NaCl at a low concentration (20 mmol) led to a significant increase in peroxidase (POD) and polyphenoloxiadase (PPO) activities, while in Jingshi-2, the PPO activity decreased progressively with an increase in NaCl concentrations. Genotype Zihuabai exhibited higher tolerance to salt stress than Jingshi-2 under in vitro conditions. These results could be used for preliminary selections of salt tolerance in potato breeding programmes.