Overexpression of AcNIP5;1, a Novel Nodulin-Like Intrinsic Protein from Halophyte Atriplex canescens, Enhances Sensitivity to Salinity and Improves Drought Tolerance in Arabidopsis

详细信息    查看全文

• 作者：Gang Yu ; Jingtao Li ; Xinhua Sun ; Xianghui Zhang…
• 关键词：Aquaporin ; Drought tolerance ; Salt sensitive ; Overexpression ; Arabidopsis thaliana
• 刊名：Plant Molecular Biology Reporter
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：33
• 期：6
• 页码：1864-1875
• 全文大小：2,890 KB
• 参考文献：Aharon R, Shahak Y, Wininger S, Bendov R, Kapulnik Y, Galili G (2003) Overexpression of a plasma membrane aquaporin in transgenic tobacco improves plant vigor under favorable growth conditions but not under drought or salt stress. Plant Cell Online 15:439–447. doi:10.​1105/​tpc.​009225 CrossRef
  Argyros RD et al (2008) Type B response regulators of Arabidopsis play key roles in cytokinin signaling and plant development. Plant Cell Online 20:2102–2116. doi:10.​1105/​tpc.​108.​059584 CrossRef
  Benzarti M, Ben Rejeb K, Debez A, Abdelly C (2013) Environmental and economical opportunities for the valorisation of the genus Atriplex: new insights. In: Hakeem KR, Ahmad P, Ozturk M (eds) Crop Improvement. Springer US, pp 441–457. doi:10.​1007/​978-1-4614-7028-1_​16
  Bienert G, Thorsen M, Schussler M, Nilsson H, Wagner A, Tamas M, Jahn T (2008) A subgroup of plant aquaporins facilitate the bi-directional diffusion of As(OH)3 and Sb(OH)3 across membranes. BMC Biol 6:26PubMed PubMedCentral CrossRef
  Cabello-Hurtado F, Ramos J (2004) Isolation and functional analysis of the glycerol permease activity of two new nodulin-like intrinsic proteins from salt stressed roots of the halophyte Atriplex nummularia. Plant Sci 166:633–640. doi:10.​1016/​j.​plantsci.​2003.​11.​001 CrossRef
  Chaumont F, Tyerman SD (2014) Aquaporins: highly regulated channels controlling plant water relations. Plant Physiol 164:1600–1618. doi:10.​1104/​pp.​ 113.​233791 PubMed PubMedCentral CrossRef
  Chaumont F, Barrieu F, Wojcik E, Chrispeels MJ, Jung R (2001) Aquaporins constitute a large and highly divergent protein family in maize. Plant Physiol 125:1206–1215. doi:10.​1104/​pp.​ 125.​3.​1206 PubMed PubMedCentral CrossRef
  Chowdhury SR, Choudhuri MA (1985) Hydrogen peroxide metabolism as an index of water stress tolerance in jute. Physiol Plant 65:476–480. doi:10.​1111/​j.​1399-3054.​1985.​tb08676.​x CrossRef
  Cui X-H, Hao F-S, Chen H, Chen J, Wang X-C (2008) Expression of the Vicia faba VfPIP1 gene in Arabidopsis thaliana plants improves their drought resistance. J Plant Res 121:207–214. doi:10.​1007/​s10265-007-0130-z PubMed CrossRef
  Danielson J, Johanson U (2008) Unexpected complexity of the Aquaporin gene family in the moss Physcomitrella patens. BMC Plant Biol 8:45PubMed PubMedCentral CrossRef
  Hu W et al (2012) Overexpression of a wheat aquaporin gene, TaAQP8, enhances salt stress tolerance in transgenic tobacco. Plant Cell Physiol 53:2127–2141. doi:10.​1093/​pcp/​pcs154 PubMed CrossRef
  Johanson U, Gustavsson S (2002) A new subfamily of major intrinsic proteins in plants. Mol Biol Evol 19:456–461PubMed CrossRef
  Johanson U et al (2001) The complete set of genes encoding major intrinsic proteins in Arabidopsis provides a framework for a new nomenclature for major intrinsic proteins in plants. Plant Physiol 126:1358–1369. doi:10.​1104/​pp.​ 126.​4.​1358 PubMed PubMedCentral CrossRef
  Kourie JI (1998) Interaction of reactive oxygen species with ion transport mechanisms. Am J Physiol Cell Physiol 275:C1–C24
  Li J, Sun X, Yu G, Jia C, Liu J, Pan H (2014a) Generation and analysis of expressed sequence tags (ESTs) from halophyte Atriplex canescens to explore salt-responsive related genes. Int J Mol Sci 15:11172–11189PubMed PubMedCentral CrossRef
  Li J, Yu G, Sun X, Jia C, Du Q, Li Q, Pan H (2014b) Modification of vectors for functional genomic analysis in plants. Genet Mol Res 13:7815–7825. doi:10.​4238/​2014.​September.​26.​20 PubMed CrossRef
  Martinez-Ballesta MC, Carvajal M (2014) New challenges in plant aquaporin biotechnology. Plant Sci 217–218:71–77. doi:10.​1016/​j.​plantsci.​2013.​12.​006 CrossRef
  Maurel C, Santoni V, Luu D-T, Wudick MM, Verdoucq L (2009) The cellular dynamics of plant aquaporin expression and functions. Curr Opin Plant Biol 12:690–698. doi:10.​1016/​j.​pbi.​2009.​09.​002 PubMed CrossRef
  Mitani-Ueno N, Yamaji N, Zhao F-J, Ma JF (2011) The aromatic/arginine selectivity filter of NIP aquaporins plays a critical role in substrate selectivity for silicon, boron, and arsenic. J Exp Bot. doi:10.​1093/​jxb/​err158 PubMed PubMedCentral
  Mittler R, Vanderauwera S, Gollery M, Van Breusegem F (2004) Reactive oxygen gene network of plants. Trends Plant Sci 9:490–498. doi:10.​1016/​j.​tplants.​2004.​08.​009 PubMed CrossRef
  Sakurai J, Ishikawa F, Yamaguchi T, Uemura M, Maeshima M (2005) Identification of 33 rice aquaporin genes and analysis of their expression and function. Plant Cell Physiol 46:1568–1577. doi:10.​1093/​pcp/​pci172 PubMed CrossRef
  Schüssler MD et al (2008) The effects of the loss of TIP1;1 and TIP1;2 aquaporins in Arabidopsis thaliana. Plant J 56:756–767. doi:10.​1111/​j.​1365-313X.​2008.​03632.​x PubMed CrossRef
  Shen B, Hohmann S, Jensen RG, Bohnert JH (1999) Roles of sugar alcohols in osmotic stress adaptation. replacement of glycerol by mannitol and sorbitol in yeast. Plant Physiol 121:45–52. doi:10.​1104/​pp.​ 121.​1.​45 PubMed PubMedCentral CrossRef
  Siefritz F, Tyree MT, Lovisolo C, Schubert A, Kaldenhoff R (2002) PIP1 plasma membrane aquaporins in tobacco: from cellular effects to function in plants. Plant Cell Online 14:869–876. doi:10.​1105/​tpc.​000901 CrossRef
  Srivastava AK, Penna S, Nguyen DV, Tran L-SP (2014) Multifaceted roles of aquaporins as molecular conduits in plant responses to abiotic stresses. Crit Rev Biotechnol 0:1–10. doi:10.​3109/​07388551.​2014.​973367
  Sun X-H et al (2014a) A heavy metal-associated protein (AcHMA1) from the halophyte, Atriplex canescens (Pursh) Nutt., confers tolerance to iron and other abiotic stresses when expressed in Saccharomyces cerevisiae. Int J Mol Sci 15:14891–14906PubMed PubMedCentral CrossRef
  Sun Y, Yan F, Cui X, Liu F (2014b) Plasticity in stomatal size and density of potato leaves under different irrigation and phosphorus regimes. J Plant Physiol 171:1248–1255. doi:10.​1016/​j.​jplph.​2014.​06.​002 PubMed CrossRef
  Tanaka M, Wallace IS, Takano J, Roberts DM, Fujiwara T (2008) NIP6;1 Is a boric acid channel for preferential transport of boron to growing shoot tissues in Arabidopsis. Plant Cell Online 20:2860–2875. doi:10.​1105/​tpc.​108.​058628 CrossRef
  Tornroth-Horsefield S et al (2006) Structural mechanism of plant aquaporin gating. Nature 439:688–694, http://​www.​nature.​com/​nature/​journal/​v439/​n7077/​suppinfo/​nature04316_​S1.​html PubMed CrossRef
  Wang X et al (2011) A novel Glycine soja tonoplast intrinsic protein gene responds to abiotic stress and depresses salt and dehydration tolerance in transgenic Arabidopsis thaliana. J Plant Physiol 168:1241–1248. doi:10.​1016/​j.​jplph.​2011.​01.​016 PubMed CrossRef
  Wang L-L et al (2014) The Thellungiella salsuginea tonoplast aquaporin TsTIP1;2 functions in protection against multiple abiotic stresses. Plant Cell Physiol 55:148–161. doi:10.​1093/​pcp/​pct166 PubMed PubMedCentral CrossRef
  Xu C, Wang M, Zhou L, Quan T, Xia G (2013) Heterologous expression of the wheat aquaporin gene TaTIP2;2 compromises the abiotic stress tolerance of Arabidopsis thaliana. PLoS One 8:e79618. doi:10.​1371/​journal.​pone.​0079618 PubMed PubMedCentral CrossRef
  Yue C et al (2014) Molecular cloning and expression analysis of tea plant aquaporin (AQP) gene family. Plant Physiol Biochem 83:65–76. doi:10.​1016/​j.​plaphy.​2014.​07.​011 PubMed CrossRef
  Zhou S et al (2012) Overexpression of the wheat aquaporin gene, TaAQP7, enhances drought tolerance in transgenic tobacco. PLoS One 7:e52439. doi:10.​1371/​journal.​pone.​0052439 PubMed PubMedCentral CrossRef
  
• 作者单位：Gang Yu (1)
  Jingtao Li (1)
  Xinhua Sun (1)
  Xianghui Zhang (1)
  Jinliang Liu (1)
  Hongyu Pan (1)
  
  1. College of Plant Sciences, Jilin University, Changchun, Jilin, 130062, People’s Republic of China
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Plant Physiology
  
• 出版者：Springer Netherlands
• ISSN：1572-9818

文摘

Aquaporins (AQPs) in plants have been shown to facilitate the transport of water and other small molecules through biological membranes. This activity is essential for plant stress management during drought. Here, we describe and characterize a novel aquaporin gene AcNIP5;1 (Atriplex canescens nodulin 26-like intrinsic protein 5;1) isolated from an A. canescens cDNA library. qRT-PCR results indicated that the transcript level in leaf tissue of AcNIP5;1 was upregulated by 20 % PEG 6000 and downregulated by 400 mM NaCl. To further understand the function of AcNIP5;1, we generated transgenic Arabidopsis thaliana plants that expressed AcNIP5;1 under control of the CaMV35S promoter. The overexpression of AcNIP5;1 in these transgenic Arabidopsis plants resulted in higher drought tolerance and sensitivity to salinity compared to wild-type plants on both MS plate and pot experiments. AcNIP5;1 protein tagged with GFP was localized to the cell plasma membrane in Nicotiana benthamiana. Our results suggest that AcNIP5;1 might play a role in drought tolerance in A. canescens. Keywords Aquaporin Drought tolerance Salt sensitive Overexpression Arabidopsis thaliana