Voltammetric studies on the interaction of epinephrine with DNA base on a glassy carbon electrode modified with Au nanoparticles
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- 作者：Xueliang Wang ; Jinjin Li ; Tao Wang ; Zhangyu Yu
- 关键词：Epinephrine ; DNA ; Interaction ; Voltammetry ; Au nanoparticles
- 刊名：Ionics
- 出版年：2015
- 出版时间：April 2015
- 年：2015
- 卷：21
- 期：4
- 页码：1105-1110
- 全文大小：868 KB
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- 刊物主题：Chemistry
Electrochemistry
Materials Science
Physical Chemistry
Condensed Matter
Renewable Energy Sources
Electrical Power Generation and Transmission- 出版者：Springer Berlin / Heidelberg
- ISSN：1862-0760
文摘
The interaction of epinephrine (EP) with DNA, including double-strand DNA (dsDNA) and single-strand DNA (ssDNA) were investigated both in Mcllvaine buffer solution and on a glassy carbon electrode (GCE) modified with Au nanoparticles (nanoAu/GCE). In pH?7.0 Mcllvaine buffer solution, EP had a sensitive oxidation current peak at 0.162?V (vs. SCE) on the nanoAu/GCE. Factors influencing the interaction in Mcllvaine buffer solution were optimized, and some electrochemical parameters, such as number of electron transferred, the standard rate constant of the electrode reaction (K s) were compared in the presence and absence of DNA. The binding ratio (m) of EP with DNA and the equilibrium constant (β) were also calculated. EP did not interact with DNA via intercalation mode.