Effects of abiotic stresses on the expression of Lhcb1 gene and photosynthesis of Oenanthe javanica and Apium graveolens

详细信息    查看全文

• 作者：Q. Jiang (1)
  Z. -S. Xu (1)
  F. Wang (1)
  M. -Y. Li (1)
  J. Ma (1)
  A. -S. Xiong (1)
  
• 关键词：chlorophyll ; light harvesting complex ; net photosysnthetic rate ; salinity ; stomatal conductance ; temperature ; transpiration rate ; water stress
• 刊名：Biologia Plantarum
• 出版年：2014
• 出版时间：June 2014
• 年：2014
• 卷：58
• 期：2
• 页码：256-264
• 全文大小：
• 参考文献：1. Andersson, J., Wentworth, M., Walters, R.G., Howard, C.A., Ruban, A.V., Horton, P., Jansson, S.: Absence of the Lhcb1 and Lhcb2 proteins of the light-harvesting complex of photosystem II 鈥?effects on photosynthesis, grana stacking and fitness. 鈥?Plant J, 35: 350鈥?61, 2003. CrossRef
  2. Bertaccini, E., Trudell, J.R.: Predicting the transmembrane secondary structure of ligand-gated ion channels. 鈥?Protein Engn. 15: 443鈥?54, 2002. CrossRef
  3. Durnford, D.G., Deane, J.A., Tan, S., McFadden, G.I., Gantt, E., Green, B.R.: A phylogenetic assessment of the eukaryotic light-harvesting antenna proteins, with implications for plastid evolution. 鈥?J. mol. Evol. 48: 59鈥?8, 1999. CrossRef
  4. Emanuelsson, O., Nielsen, H., Von Heijne, G.: ChloroP, a neural network-based method for predicting chloroplast transit peptides and their cleavage sites. 鈥?Protein Sci. 8: 978鈥?84, 1999. CrossRef
  5. Galka, P., Santabarbara, S., Khuong, T.T., Degand, H., Morsomme, P., Jennings, R.C., Boekema, E.J., Caffarri, S.: Functional analyses of the plant photosystem I-lightharvesting complex II supercomplex reveal that lightharvesting complex II loosely bound to photosystem II is a very efficient antenna for photosystem I in state II. 鈥?Plant Cell 24: 2963鈥?978, 2012. CrossRef
  6. Ganeteg, U., Kulheim, C., Andersson, J., Jansson, S.: Is each light-harvesting complex protein important for plant fitness? 鈥?Plant Physiol. 134: 502鈥?09, 2004. CrossRef
  7. Gasteiger, E., Gattiker, A., Hoogland, C., Ivanyi, I., Appel, R.D., Bairoch, A.: ExPASy: the proteomics server for in-depth protein knowledge and analysis. 鈥?Nucl. Acids Res. 31: 3784鈥?788, 2003. CrossRef
  8. Green, B.R., Durnford, D.G.: The chlorophyll-carotenoid proteins of oxygenic photosynthesis. 鈥?Annu. Rev. Plant Physiol. Plant mol. Biol. 47: 685鈥?14, 1996. CrossRef
  9. Guo, P., Baum, M., Grando, S., Ceccarelli, S., Bai, G., Li, R., von Korff, M., Varshney, R.K., Graner, A., Valkoun, J.: Differentially expressed genes between drought-tolerant and drought-sensitive barley genotypes in response to drought stress during the reproductive stage. 鈥?J. exp. Bot. 60: 3531鈥?544, 2009. CrossRef
  10. Hazen, S.P., Pathan, M.S., Sanchez, A., Baxter, I., Dunn, M., Estes, B., Chang, H.S., Zhu, T., Kreps, J.A., Nguyen, H.T.: Expression profiling of rice segregating for drought tolerance QTLs using a rice genome array. 鈥?Funct. Integr. Genomics 5: 104鈥?16, 2005. CrossRef
  11. Ho, B.K., Brasseur, R.: The Ramachandran plots of glycine and pre-proline. 鈥?BMC Struct. Biol. 5: 14, 2005. CrossRef
  12. Hobe, S., Forster, R., Klingler, J., Paulsen, H.: N-proximal sequence motif in light-harvesting chlorophyll / a/ / b-binding protein is essential for the trimerization of light-harvesting chlorophyll a/b complex. 鈥?Biochemistry 34: 10224鈥?0228, 1995. CrossRef
  13. Humbeck, K., Krupinska, K.: The abundance of minor chlorophyll / a/ / b-binding proteins CP29 and LHCI of barley ( / Hordeum vulgare L.) during leaf senescence is controlled by light. 鈥?J. exp. Bot. 54: 375鈥?83, 2003. CrossRef
  14. Iovene, M., Grzebelus, E., Carputo, D., Jiang, J., Simon, P.W.: Major cytogenetic landmarks and karyotype analysis in / Daucus carota and other / Apiaceae. 鈥?Amer. J. Bot. 95: 793鈥?04, 2008. CrossRef
  15. Jansson, S.: A guide to the / Lhc genes and their relatives in / Arabidopsis. 鈥?Trends Plant Sci. 4: 236鈥?40, 1999. CrossRef
  16. Kiefer, F., Arnold, K., Kunzli, M., Bordoli, L., Schwede, T.: The Swiss-Model repository and associated resources. 鈥?Nucl. Acids Res. 37: D387鈥?92, 2009. CrossRef
  17. Kim, D.Y., Hong, M.J., Lee, Y.J., Lee, M.B., Seo, Y.W.: Wheat truncated hemoglobin interacts with photosystem I PSK-I subunit and photosystem II subunit PsbS1. 鈥?Biol. Plant. 57: 281鈥?90, 2013. CrossRef
  18. Kovacs, L., Damkjaer, J., Kereiche, S., Ilioaia, C., Ruban, A.V., Boekema, E.J., Jansson, S., Horton, P.: Lack of the lightharvesting complex CP24 affects the structure and function of the grana membranes of higher plant chloroplasts. 鈥?Plant Cell 18: 3106鈥?120, 2006. CrossRef
  19. Li, Y.H., Liu, Y.J., Xu, X.L., Jin, M., An, L.Z., Zhang, H.: Effect of 24-epibrassinolide on drought stress-induced changes in / Chorispora bungeana. 鈥?Biol. Plant. 56: 192鈥?96, 2012. CrossRef
  20. Liang, X., Qiao, D., Huang, M., Yi, X., Bai, L., Xu, H., Wei, L., Zeng, J., Cao, Y.: Identification of a gene encoding the lightharvesting chlorophyll / a/ / b proteins of photosystem I in green alga / Dunaliella salina. 鈥?DNA Sequence 19: 137鈥?45, 2008.
  21. Liu, Z., Yan, H., Wang, K., Kuang, T., Zhang, J., Gui, L., An, X., Chang, W.: Crystal structure of spinach major lightharvesting complex at 2.72 脜 resolution. 鈥?Nature 428: 287鈥?92, 2004. CrossRef
  22. Loukehaich, R., Wang, T., Ouyang, B., Ziaf, K., Li, H., Zhang, J., Lu, Y., Ye, Z.: SpUSP, an annexin-interacting universal stress protein, enhances drought tolerance in tomato. 鈥?J. exp. Bot. 63: 5593鈥?606, 2012. CrossRef
  23. Manickavelu, A., Kawaura, K., Oishi, K., Shin, I.T., Kohara, Y., Yahiaoui, N., Keller, B., Suzuki, A., Yano, K., Ogihara, Y.: Comparative gene expression analysis of susceptible and resistant near-isogenic lines in common wheat infected by / Puccinia triticina. 鈥?DNA Res. 17: 211鈥?22, 2010. CrossRef
  24. Nakashima, K., Yamaguchi-Shinozaki, K.: ABA signaling in stress-response and seed development. 鈥?Plant Cell Rep. 32: 959鈥?70, 2013. CrossRef
  25. Nott, A., Jung, H.S., Koussevitzky, S., Chory, J.: Plastid-tonucleus retrograde signaling. 鈥?Annu. Rev. Plant Biol. 57: 739鈥?59, 2006. CrossRef
  26. Nussberger, S., Dorr, K., Wang, D.N., Kuhlbrandt, W.: Lipidprotein interactions in crystals of plant light-harvesting complex. 鈥?J. mol. Biol. 234: 347鈥?56, 1993. CrossRef
  27. Pfaffl, M.W.: A new mathematical model for relative quantification in real-time RT-PCR. 鈥?Nucl. Acids Res. 29: e45, 2001. CrossRef
  28. Seki, M., Narusaka, M., Ishida, J., Nanjo, T., Fujita, M., Oono, Y., Kamiya, A., Nakajima, M., Enju, A., Sakurai, T., Satou, M., Akiyama, K., Taji, T., Yamaguchi-Shinozaki, K., Carninci, P., Kawai, J., Hayashizaki, Y., Shinozaki, K.: Monitoring the expression profiles of 7000 / Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray. 鈥?Plant J. 31: 279鈥?92, 2002. CrossRef
  29. Staneloni, R.J., Rodriguez-Batiller, M.J., Casal, J.J.: Abscisic acid, high-light, and oxidative stress down-regulate a photosynthetic gene via a promoter motif not involved in phytochrome-mediated transcriptional regulation. 鈥?Mol. Plants 1: 75鈥?3, 2008. CrossRef
  30. Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., Kumar, S.: MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. 鈥?Mol. Biol. Evol. 28: 2731鈥?739, 2011. CrossRef
  31. Teramoto, H., Ono, T., Minagawa, J.: Identification of / Lhcb gene family encoding the light-harvesting chlorophyll / a/ / b proteins of photosystem II in / Chlamydomonas reinhardtii. 鈥?Plant Cell Physiol. 42: 849鈥?56, 2001. CrossRef
  32. Wei, L., Cao, Y., Liang, X., Liu, Y., Deng, T., Bai, L., Qiao, D.: Identification of two genes encoding the major lightharvesting chlorophyll / a/ / b proteins of photosystem II in green alga / Dunaliella salina. 鈥?DNA Sequence 17: 370鈥?77, 2006.
  33. Xia, Y., Ning, Z., Bai, G., Li, R., Yan, G., Siddique, K.H., Baum, M., Guo, P.: Allelic variations of a light harvesting chlorophyll / a/ / b-binding protein gene ( / Lhcb1) associated with agronomic traits in barley. 鈥?PLoS One 7: e37573, 2012. CrossRef
  34. Xu, Y.H., Liu, R., Yan, L., Liu, Z.Q., Jiang, S.C., Shen, Y.Y., Wang, X.F., Zhang, D.P.: Light-harvesting chlorophyll / a/ / bbinding proteins are required for stomatal response to abscisic acid in / Arabidopsis. 鈥?J. exp. Bot. 63: 1095鈥?106, 2012. CrossRef
  35. Xue, W., Li, X.Y., Zhu, J.T., Lin, L.S.: Effects of temperature and irradiance on photosystem activity during / Alhagi sparsifolia leaf senescence. 鈥?Biol. Plant. 56: 785鈥?88, 2012. CrossRef
  36. Yamaguchi-Shinozaki, K., Shinozaki, K.: Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses. 鈥?Annu Rev. Plant Biol. 57: 781鈥?03, 2006. CrossRef
  37. Zhao, D., Yan, Z.M., Zhang, S.N., Li, J.X., Liu, H.J.: Karyotype analysis of main umbelliferous vegetables. 鈥?Acta bot. boreal -occident. sin. 30: 1978鈥?981, 2010.
  
• 作者单位：Q. Jiang (1)
  Z. -S. Xu (1)
  F. Wang (1)
  M. -Y. Li (1)
  J. Ma (1)
  A. -S. Xiong (1)
  
  1. State Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Agriculture and College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, P.R. China
  
• ISSN：1573-8264

文摘

The effects of abiotic stresses on the expression of Lhcb1 gene (coding light-harvesting chlorophyll-protein complex II), and on photosynthetic rate were studied in one Oenanthe javanica (cv. Baguazhou Shuiqin) and three Apium graveolens cultivars (Liuhe Huangxinqin, Jinnan Shiqin, and Ventura). The Lhcb1 genes were cloned and we predicted that Lhcb1 proteins were most probably able to form homo-trimers. Each monomer contained five helical segments, of which three were likely transmembrane helices, and 15 putative chlorophyll-binding sites. The abiotic stresses affected the A. graveolens similarly in all the cultivars, however, the O. javanica photosynthesis was not significantly affected. The expression of the Lhcb1 gene was up-regulated under the cold, heat, salt, and drought stresses in the cvs Liuhe Huangxinqin and Jinnan Shiqin. The Lhcb1 was up-regulated under the heat, salt, and drought stresses in the cv. Ventura, whereas had no significant changes under the cold stress. No significant changes of the Lhcb1 expression were found under the cold and salt stresses and even little down-regulation following the heat and drought stresses in Oenanthe javanica. The expression of Lhcb1 may be a useful indicator of photosynthetic activity.