Development of a two-step process for production of 3-hydroxypropionic acid from glycerol using Klebsiella pneumoniae and Gluconobacter oxydans

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• 作者：Li Zhao ; Jinping Lin ; Hualei Wang ; Jingli Xie…
• 关键词：3 ; Hydroxypropionic acid ; Glycerol ; Klebsiella pneumoniae ; Gluconobacter oxydans ; Two ; step process
• 刊名：Bioprocess and Biosystems Engineering
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：38
• 期：12
• 页码：2487-2495
• 全文大小：475 KB
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  24.Cassandra DM, Catarina SSP, Myriam N, Sofie P, Wim S, Erick JV (
• 作者单位：Li Zhao (1)
  Jinping Lin (1)
  Hualei Wang (1)
  Jingli Xie (1)
  Dongzhi Wei (1)
  
  1. State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai, 200237, People’s Republic of China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Industrial Chemistry and Chemical Engineering
  Industrial and Production Engineering
  Waste Management and Waste Technology
  Waste Water Technology, Water Pollution Control, Water Management and Aquatic Pollution
  Food Science
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1615-7605

文摘

In this work, a two-step process was developed for the production of 3-hydroxypropionic acid from glycerol. In the first step, glycerol was converted to 1,3-propanediol by Klebsiella pneumonia. In the second step, the 1,3-propanediol was converted into 3-hydroxypropionic acid by Gluconobacter oxydans. In a 7.0 L bioreactor, the whole process took 54 h, consumed 480 g glycerol and produced 242 g 3-hydroxypropionic acid. The conversion rate of glycerol to 3-hydroxypropionic acid was 50.4 % (g g?). The final concentration of 3-hydroxypropionic acid arrived 60.5 g L?. The process was effective for 3-HP production from glycerol and it might provide a new approach to the biosynthesis of 3-HP from a cheap starting material. Moreover, in this paper, it was first reported that the by-product of 3-hydroxypropionic acid production from 1,3-propandeiol was acrylic acid. Keywords 3-Hydroxypropionic acid Glycerol Klebsiella pneumoniae Gluconobacter oxydans Two-step process