Adverse effect of DEHP exposure on the serum insulin level of Balb/c mice

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• 作者：Jing Wang ; Jinquan Li ; Kashif Rafiq Zahid ; Kun Wang…
• 关键词：DEHP exposure ; Diabetes ; like symptom ; Insulin ; Blood glucose ; Pancreatic cell ; Oxidative stress
• 刊名：Molecular & Cellular Toxicology
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：12
• 期：1
• 页码：83-91
• 全文大小：8,115 KB
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• 作者单位：Jing Wang (1) (2)
  Jinquan Li (1)
  Kashif Rafiq Zahid (1)
  Kun Wang (3)
  Yan Qian (4)
  Ping Ma (5)
  Shumao Ding (1)
  Xu Yang (1) (5)
  Xianliang Wang (4)
  
  1. School of Life Sciences, Central China Normal University, Wuhan, 430079, China
  2. Hubei Center of Diseases Control and Prevention, Wuhan, 430079, China
  3. Department of Population Health, New York University, New York, 10016, USA
  4. Institute of Environmental Health and Related Product Safety, Chinese Centre for Disease Control and Prevention, Beijing, 100021, China
  5. College of Basic Medical Science, Hubei University of Science and Technology, Xianning, 437100, China
  
• 刊物主题：Cell Biology; Pharmacology/Toxicology;
• 出版者：Springer Netherlands
• ISSN：2092-8467

文摘

Di(2-ethylhexyl) phthalate (DEHP) is a common indoor pollutant in the world, which may cause lots of harmful effects in human including diabetes according to epidemiological studies. To explore the underlying role of DEHP in diabetes-like symptoms, Balb/c mice were chose to be the experimental animals in this paper. They were separated as eight groups as follows: (1) saline+normal diet (vehicle control), (2) 10 mg/kg.day DEHP+normal diet, (3) 50 mg/kg.day DEHP+normal diet, (4) 250 mg/kg.day DEHP+normal diet, (5) streptozotocin (STZ)+high fat diet (diabetes model), (6) 10 mg/kg.day DEHP+ STZ+high fat diet, (7) 50 mg/kg.day DEHP+STZ +high fat diet, (8) 250 mg/kg.day DEHP+STZ+high fat diet. The biomarkers of this experiment include four groups: (1) general indicates: body weight and drinking water, (2) blood biomarkers: serum insulin and fasting glucose, (3) pathological examination: pancreas section and kidney section, and (4) biomarkers of oxidative stress: reactive oxygen species (ROS) and malondialdehyde (MDA) in liver cells. Our study results demonstrate that: (1) at our treatment levels DEHP cannot directly induce diabetes, but reduce serum insulin level in DEHP-exposed non-STZ-treated animals, (2) pathological examination finds that these is a dose-dependent damage in the pancreas in DEHP- exposed STZ-treated groups, and (3) the oxidative mechanism may be involved in this pathological process.