Using high-throughput sequencing to assess the impacts of treated and untreated wastewater discharge on prokaryotic communities in an urban river

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• 作者：Yaohui Bai (1)
  Weixiao Qi (1)
  Jinsong Liang (1)
  Jiuhui Qu (1)
  
• 关键词：Effluent ; dominated river ; Wastewater discharge ; Prokaryotic community ; High ; throughput sequencing ; Community composition and function
• 刊名：Applied Microbiology and Biotechnology
• 出版年：2014
• 出版时间：February 2014
• 年：2014
• 卷：98
• 期：4
• 页码：1841-1851
• 全文大小：434 KB
• 作者单位：Yaohui Bai (1)
  Weixiao Qi (1)
  Jinsong Liang (1)
  Jiuhui Qu (1)
  
  1. State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, People鈥檚 Republic of China
  
• ISSN：1432-0614

文摘

In many megacities wastewater is an important source of surface water, particularly during drought periods. While changes in surface water chemistry associated with effluent inflow have generally been well-studied, few data have been collected on the effects to prokaryotic communities. The objective of this study was to explore the impacts of treated and untreated wastewater discharges on prokaryotic community in an urban river. High-throughput sequencing was conducted for analyzing the prokaryotic community composition and function in river water, treated wastewater and untreated wastewater. Results revealed that the prokaryotic community compositions in the upstream river reach were dominated by treated wastewater discharge. In the middle- and downstream river reaches, untreated effluent volumes are higher, thus affecting the structure of the prokaryotic community, promoting a rise in Cyanobacteria and Thaumarchaeota. Function annotation revealed a number of genes associated with xenobiotic metabolism and human diseases were observed in river and wastewater samples, suggesting wastewater discharge to river may pose a risk to human health. Quantitative real-time PCR results revealed that the treated and untreated wastewater discharges also affected the abundance of ammonia oxidation bacteria (AOB) and ammonia oxidation archaea (AOA) in river.