The effect of Survivin antioligonucleotide on the apoptosis of Xuanwei lung adenocarcinoma cell

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• 作者：Weiwei Wang (1)
  Gaofeng Li (1)
  Zhipeng Hong (2)
  Ruibing Cheng (1)
  Jipeng Zhang (1)
  Lianhua Ye (1)
  
• 关键词：Survivin ; antioligonucleotide ; XWLC ; 05 ; apoptosis
• 刊名：The Chinese-German Journal of Clinical Oncology
• 出版年：2011
• 出版时间：May 2011
• 年：2011
• 卷：10
• 期：5
• 页码：252-255
• 全文大小：336KB
• 参考文献：1. Vegran F, Boidot R, Oudin C, / et al. Distinct expression of Survivin splice variants in breast carcinomas. Int J Oncol, 2005, 27: 1151鈥?157.
  2. Olie RA, Sim玫es-W眉st AP, Baumann B, / et al. A novel antisense oligonucleotide targeting survivin expression induces apoptosis and sensitizes lung cancer cells to chemotherapy. Cancer Res, 2000, 60: 2805鈥?809.
  3. Xiao YP, Lin Z, Mao LL, / et al. Significance and expression of Bax, Survivin and p53 in gastric carcinoma and precancerous lesions using tissue microarray. Chinese-German J Clin Oncol, 2007, 6: 302鈥?04. CrossRef
  4. Liao L, Li ZY. Correlation between gene silencing activity and structural features of antisense oligodeoxynucleotides and target RNA. In Silico Bio, 2007, 7: 527鈥?34.
  5. Meng R, Wu G, Cheng J, / et al. The effect of antisense oligodeoxynucleotides targeting Aurora A kinase on cell proliferation and chemosensitivity to paclitaxel in human lung cancer cell line A549. Chinese-German J Clin Oncol, 2007, 6: 258鈥?63. CrossRef
  
• 作者单位：Weiwei Wang (1)
  Gaofeng Li (1)
  Zhipeng Hong (2)
  Ruibing Cheng (1)
  Jipeng Zhang (1)
  Lianhua Ye (1)
  
  1. Departmen of Thoracic Surgery, The 3rd Affiliated Hospital of Kunming Medical University, Yunnan Cancer Institute of Lung Cancer Research Center, Kunming, 650118, China
  2. Department of Thoracicsurgery, The 1st Affiliated Hospital of Kunming Medical University, Kunming, 650032, China
  

文摘

Objective The aim of the study was to investigate the effects of Survivin antioligonucleotide on the proliferation, apoptosis and cell cycle of Xuanwei lung adenocarcinoma cell XWLC-05. Methods Specific targeting Survivin ASODN was composed firstly. XWLC-05 would be divided into 4 groups: Group Sham (blank), Group Lip (simple liposome), Group Lip-SODN (transfected sense oligonucleotide) and Group Lip-ASODN (transfected ASODN). All groups had been transfected under the same condition for 48 hours. Then West blotting was used to check the expression of Survivin in cells from different groups and flow cytometer was used to find out the apoptosis rate of cells in different groups. Results The expression of XWLC-05 Survivin in Group Lip-ASODN decreased obviously and apoptosis rate was significantly higher than that of other groups (P < 0. 05). Conclusion XWLC-05 transfected by Survivin ASODN could down regulate the expression of Survivin and lower expression of Survivin might lead to the apoptosis of XWLC-05 and restrain the proliferation of cancer cells.