Identification of tyrosine 71 as a critical residue for the cytotoxic activity of Clostridium perfringens epsilon toxin towards MDCK cells

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• 作者：Zhigang Jiang (1)
  Jitao Chang (1)
  Fang Wang (1)
  Li Yu (1)
  
  1. Division of Livestock Infectious Diseases ; State Key Laboratory of Veterinary Biotechnology ; Harbin Veterinary Research Institute ; Chinese Academy of Agricultural Sciences ; 427 Maduan Street ; Harbin ; 150001 ; P. R. China
  
• 关键词：Clostridium perfringens ; epsilon toxin ; tyrosine 71 ; aromatic ring ; cytotoxic activity
• 刊名：Journal of Microbiology
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：53
• 期：2
• 页码：141-146
• 全文大小：511 KB
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  3. Borrmann, E., Gunther, H., and Kohler, H. 2001. Effect of / Clostridium perfringens epsilon toxin on MDCK cells. / FEMS Immunol. Med. Microbiol. 31, 85鈥?2. CrossRef
  4. Cole, A.R., Gibert, M., Popoff, M., Moss, D.S., Titball, R.W., and Basak, A.K. 2004. / Clostridium perfringens epsilon-toxin shows structural similarity to the pore-forming toxin aerolysin. / Nat. Struct. Mol. Biol. 11, 797鈥?98. CrossRef
  5. Ivie, S.E. and McClain, M.S. 2012. Identification of amino acids important for binding of / Clostridium perfringens epsilon toxin to host cells and to HAVCR1. / Biochemistry 51, 7588鈥?595. CrossRef
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  8. McDonel, J.L. 1986. Toxins of / Clostridium perfringens types A, B, C, D and E, pp. 477鈥?17. / In F. Dorner, J.D. (ed.), Pharmacology of bacterial toxins. Pergamon Press, Oxford.
  9. Minami, J., Katayama, S., Matsushita, O., Matsushita, C., and Okabe, A. 1997. Lambda-toxin of / Clostridium perfringens activates the precursor of epsilon-toxin by releasing its N- and C-terminal peptides. / Microbiol. Immunol. 41, 527鈥?35. CrossRef
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• 刊物主题：Microbiology;
• 出版者：Springer Netherlands
• ISSN：1976-3794

文摘

Clostridium perfringens epsilon toxin (Etx) is an extremely potent toxin, causing fatal enterotoxaemia in many animals. Several amino acids in domains I and II have been proposed to be critical for Etx to interact with MDCK cells. However, the critical amino acids in domain III remain undefined. Therefore, we assessed the effects of aromatic amino acids in domain III on Etx activity in this study. All of the results indicated that Y71 was critical for the cytotoxic activity of Etx towards MDCK cells, and this activity was dependent on the existence of an aromatic ring residue in position 71. Additionally, mutations in Y71 did not affect the binding of Etx to MDCK cells, indicating that Y71 is not a receptor binding site for Etx. In summary, we identified an amino acid in domain III that is important for the cytotoxic activity of Etx, thereby providing information on the structure-function relationship of Etx.