Isolation of two Chinese bovine enteroviruses and sequence analysis of their complete genomes
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- 作者：Yingli Li (1)
Jitao Chang (1)
Qian Wang (1)
Li Yu (1) - 刊名：Archives of Virology
- 出版年：2012
- 出版时间：December 2012
- 年：2012
- 卷：157
- 期：12
- 页码：2369-2375
- 全文大小：290KB
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Jitao Chang (1)
Qian Wang (1)
Li Yu (1)
1. State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People’s Republic of China - ISSN：1432-8798
文摘
In this study, RNA corresponding to bovine enterovirus (BEV) was detected in 24.6?% of faecal samples (17/69) from diarrheic and healthy cattle in six different areas in China by an RT-PCR screening method. Furthermore, two cytopathic agents, designated as BHM26 and BJ50, were isolated from the bovine diarrheic fecal samples. During passage in MA104 cells, ultrathin sections of virus-infected monolayers were examined using a transmission electron microscope, and a large number of symmetrical virus crystals were seen in the cytoplasm, with monomorphic small viral particles of 27-30?nm in diameter. The full-length RNA genomes were 7433 and 7416 nucleotides long, respectively, with a genome organization analogous to that of picornaviruses. Phylogenetic analysis of the VP1 and VP3 capsid protein coding sequences suggested that the viruses BHM26 and BJ50 belong to genotype 2 of the BEV cluster B (BEV-B). In addition, sequence comparisons of the 5-and 3-UTRs and P1, P2 and P3 subgenomic regions of the two isolates suggested that there were intergenotypic recombination events occurring during evolution of the BHM26 and BJ50 isolates.