Biological Removal of Phosphate at Low Concentrations Using Recombinant Escherichia coli Expressing Phosphate-Binding Protein in Periplasmic Space

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• 作者：Suk Soon Choi (1)
  Hyun Min Lee (1)
  Jeong Hyub Ha (2)
  Dong Gyun Kang (3)
  Chang Sup Kim (3)
  Jeong Hyun Seo (3)
  Hyung Joon Cha (3)
  
• 关键词：Phosphate removal ; Phosphate ; binding protein ; Escherichia coli ; Periplasmic secretion ; Whole cells
• 刊名：Applied Biochemistry and Biotechnology
• 出版年：2013
• 出版时间：November 2013
• 年：2013
• 卷：171
• 期：5
• 页码：1170-1177
• 全文大小：
• 作者单位：Suk Soon Choi (1)
  Hyun Min Lee (1)
  Jeong Hyub Ha (2)
  Dong Gyun Kang (3)
  Chang Sup Kim (3)
  Jeong Hyun Seo (3)
  Hyung Joon Cha (3)
  
  1. Department of Biological and Environmental Engineering, Semyung University, Jecheon, 390-711, Korea
  2. School of Environmental Science and Technology, Pohang University of Science and Technology, Pohang, 790-784, Korea
  3. Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, 790-784, Korea
  
• ISSN：1559-0291

文摘

During wastewater treatment, phosphate removal is an important and challenging process; thus, diverse technologies, including those derived from biological means, have been devised for efficient phosphate removal. Although conventional biological methods are effective in decreasing wastewater phosphate levels to ~1mg/L, long periods of microbial adaptation are required for effective phosphate removal, and the removal efficiency of these methods is relatively poor at lower phosphate concentrations. In the present work, we constructed a recombinant Escherichia coli with periplasmic-expressed phosphate-binding protein (PBP) and investigated its biological removal ability for low phosphate levels. We found that the PBP-expressing recombinant E. coli cells showed efficient (> 94%) removal of phosphate at low concentrations (0.2.0mg/L) in a treated cell mass-dependent manner. Collectively, we propose that our PBP-expressing recombinant whole-cell system could be successfully used during wastewater treatment for the biological removal of low concentrations of phosphate.